4′,6-Diamidino-2-phenylindole dihydrochloride

• 产品号: 32670
• CAS号: 28718-90-3
• 分子式: C16H17Cl2N5
• 分子量: 350.24568
• 纯 度: ≥95.0% (HPLC)

产品别名

• 标题: 4′,6-Diamidino-2-phenylindole dihydrochloride
• IUPAC标准名: 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
• IUPAC传统名: DAPI dihydrochloride
• 别名: DAPI dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride

产品登记号

• EC号: 249-186-7
• CAS号: 28718-90-3
• PubChem SID: 24859612
• MDL号: MFCD00012681
• Beilstein号: 4894417

产品性质

• Empirical Formula (Hill Notation): C16H15N5 · 2HCl
• 产品线: BioReagent
• 纯度: ≥95.0% (HPLC)
• 适用性: suitable for fluorescence
• 荧光: λex 340 nm; λem 488 nm (nur DAPI)
• 荧光: λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
• 荧光: λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
• 溶解度: DMF: soluble
• 溶解度: H2O: soluble
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

详细说明 (简体中文)

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.