2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride

• ChemBase编号: 104891
• 分子式: C16H17Cl2N5
• 平均质量: 350.24568
• 单一同位素质量: 349.08610093
• SMILES: Cl.Cl.NC(=N)c1ccc(cc1)c1cc2c([nH]1)cc(cc2)C(=N)N
• Canonical SMILES: NC(=N)c1ccc(cc1)c1cc2c([nH]1)cc(cc2)C(=N)N.Cl.Cl
• InChI: InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChIKey: FPNZBYLXNYPRLR-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
• IUPAC传统名: DAPI dihydrochloride
• 别名: DAPI; 4',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride; 4′,6-Diamidino-2-phenylindole dihydrochloride

登记号

• CAS号: 28718-90-3
• EC号: 249-186-7
• MDL号: MFCD00012681
• Beilstein号: 4894417
• PubChem SID: 162091779; 24894305; 24859612; 24894213
• PubChem CID: 160166

理论计算性质

• Acid pKa: 14.501453
• 质子受体: 4
• 质子供体: 5
• LogD (pH = 5.5): -3.3503847
• LogD (pH = 7.4): -3.3225315
• Log P: 1.4801712
• 摩尔折射率: 105.5032 cm^3
• 极化性: 33.95792 Å^3
• 极化表面积: 115.53 Å^2
• 可自由旋转的化学键: 3
• 里宾斯基五规则: true

分子性质

理化性质

• 溶解度: DMF: soluble; H2O: soluble; H2O: soluble20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.); H2O: soluble20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.); PBS: insoluble
• 外观: powder
• 紫外吸收波长: ε/263 nm, H2O 30; ε1mM/263 nm, H2O 30
• 荧光: λex 340 nm; λem 488 nm (nur DAPI); λex 364 nm; λem 454 nm (DAPI-DNA-Komplex); λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

安全信息

• 保存条件: 0°C, Desiccate, Store Under Nitrogen, Protect from light
• 德国WGK号: 3
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 保存温度: 2-8°C

产品相关信息

• 纯度: ≥95.0% (HPLC); ≥98% (HPLC and TLC)
• 适用性: suitable for cell culture; suitable for fluorescence
• 产品质量级别: PREMIUM
• 产品线: BioReagent
• Empirical Formula (Hill Notation): C16H15N5 · 2HCl

详细说明

MP Biomedicals - 02157574

Dihydrochloride
Crystalline

Sigma Aldrich - D9542

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Caution
Protect from light.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Sigma Aldrich - D8417

Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Caution
Protect from light.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Sigma Aldrich - 32670

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.