Cholera Toxin B subunit

• 产品号: C9972
• 分子式: C16H13ClF3NO2
• 分子量: 343.7281296

产品别名

• 标题: Cholera Toxin B subunit
• IUPAC标准名: N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
• IUPAC传统名: N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
• 别名: CTB

产品登记号

• MDL号: MFCD02356522

产品性质

• 主要成分: Protein, ~40% Lowry
• Conjugate: biotin conjugate
• Mol. Weight: mol wt ~12 kDa
• 外观: lyophilized powder
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 保存温度: 2-8°C
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Quality
Biotin content ~1.0 mole/mole protein.
Physical form
Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

详细说明 (简体中文)

Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Quality
Biotin content ~1.0 mole/mole protein.
Physical form
Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.