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MFCD02356522 分子结构
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N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide

ChemBase编号:133942
分子式:C16H13ClF3NO2
平均质量:343.7281296
单一同位素质量:343.058691
SMILES和InChIs

SMILES:
CCOc1ccccc1C(=O)Nc1ccc(c(c1)C(F)(F)F)Cl
Canonical SMILES:
CCOc1ccccc1C(=O)Nc1ccc(c(c1)C(F)(F)F)Cl
InChI:
InChI=1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
InChIKey:
YDXZSNHARVUYNM-UHFFFAOYSA-N

引用这个纪录

CBID:133942 http://www.chembase.cn/molecule-133942.html

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名称和登记号

名称和登记号

名称 登记号
IUPAC标准名
N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
IUPAC传统名
N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
别名
CTB
Cholera Toxin B subunit
MDL号
MFCD02356522
PubChem SID
162228219
PubChem CID
729859

数据来源

数据来源

所有数据来源 商品来源 非商品来源
数据来源 数据ID
PubChem 729859 external link

理论计算性质

理论计算性质

JChem
Acid pKa 10.602757  质子受体
质子供体 LogD (pH = 5.5) 4.746157 
LogD (pH = 7.4) 4.745902  Log P 4.7461605 
摩尔折射率 83.5818 cm3 极化性 30.326454 Å3
极化表面积 38.33 Å2 可自由旋转的化学键
里宾斯基五规则 true 

分子性质

分子性质

理化性质 安全信息 产品相关信息 生物活性(PubChem)
溶解度
H2O: soluble10 mg/mL expand 查看数据来源
外观
lyophilized powder expand 查看数据来源
tan lyophilized powder expand 查看数据来源
white solid expand 查看数据来源
欧盟危险性物质标志
B expand 查看数据来源
刺激性(Irritant) 刺激性(Irritant) (Xi) expand 查看数据来源
有害性(Harmful) 有害性(Harmful) (Xn) expand 查看数据来源
MSDS下载
下载链接 expand 查看数据来源
下载链接 expand 查看数据来源
下载链接 expand 查看数据来源
下载链接 expand 查看数据来源
下载链接 expand 查看数据来源
下载链接 expand 查看数据来源
德国WGK号
2 expand 查看数据来源
3 expand 查看数据来源
危险公开号
20/21/22-36/37/38-52/53 expand 查看数据来源
36/38 expand 查看数据来源
安全公开号
26-36 expand 查看数据来源
个人保护装置
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter expand 查看数据来源
保存温度
2-8°C expand 查看数据来源
纯度
≥95% (SDS-PAGE) expand 查看数据来源
主要成分
Protein, ~20% Lowry expand 查看数据来源
Protein, ~40% Lowry expand 查看数据来源
Protein, ~5% Lowry expand 查看数据来源
杂质
≤0.5% Cholera toxin A subunit (SDS-PAGE) expand 查看数据来源
标签扩展内容
~1.0 mol FITC per mol protein expand 查看数据来源
产品质量级别
PREMIUM expand 查看数据来源
Mol. Weight
mol wt ~12 kDa expand 查看数据来源
pentamer mol wt 57 kDa expand 查看数据来源
Capacity
>30 units/μg, protein antitoxin combining activity (toxoid)(Lowry) expand 查看数据来源
permeability factor (PF) activity
<0.05% expand 查看数据来源
Conjugate
biotin conjugate expand 查看数据来源
FITC conjugate expand 查看数据来源
peroxidase conjugate expand 查看数据来源
peroxidase conjugate (Contains ~ 2 moles HRP/mole of CTB. ~100 μg HRP conjugated to ~45 μg CTB) expand 查看数据来源
peroxidase activity
>100 U/mg, pH 6.0, 20 °C expand 查看数据来源

详细说明

详细说明

Sigma Aldrich Sigma Aldrich
Sigma Aldrich -  C9903 external link
Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
包装
Package size based on protein content
Reconstitution
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Sigma Aldrich -  C3741 external link
Application
Cholera toxin B subunit conjugated to peroxidase (CB-HRP) has been shown to be a more effective retrograde and transganglionic tracer for pelvic primary afferents from the urinary bladder than wheat germ agglutinin-horseradish peroxidase or isolectin B4-horseradish peroxidase. Retrograde labeling with CB-HRP has been demonstrated in symphathetic pre-ganglionic neurons as well as in cells of the fastigial nuclei. It is also an effective anterograde tracer for the study of axonal and terminal labeling in brain stem.
Caution
Do not freeze.
包装
Prepared and packaged using aseptic technique and sealed under vacuum.
Reconstitution
Reconstitution of the vial with 100 μl of water will yield a final solution containing: 1 mg/mL HRP, 0.45 mg/ml CTB, 10 mM phosphate buffer and 150 mM NaCl. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be sterile filtered through a 0.2 μm filter. Store the reconstituted solutions at 2-8 °C.
Specificity
HRP activity exceedes 100 pyrogallol units per mg of HRP in the CTB-HRP conjugate. Activity: 10-30 pyrogallol units per vial.
Unit Definition
Unit definition: One pyrogallol unit converts 1 mg of pyrogallol to purpurogallin in 20 seconds at pH 6.0 at 20 °C.
Physical form
Lyophilized powder containing 11-13% protein with the balance consisting of phosphate buffer and sodium chloride.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Sigma Aldrich -  C4672 external link
Application
Cholera toxin B subunit conjugated to peroxidase (CB-HRP) has been shown to be a more effective retrograde and transganglionic tracer for pelvic primary afferents from the urinary bladder than wheat germ agglutinin-horseradish peroxidase or isolectin B4-horseradish peroxidase. Retrograde labeling with CB-HRP has been demonstrated in symphathetic pre-ganglionic neurons as well as in cells of the fastigial nuclei. It is also an effective anterograde tracer for the study of axonal and terminal labeling in brain stem.
包装
Vial contains approx. 42 μg cholera toxin B subunit, conjugated to 100 μg (10-30 units) horseradish peroxidase, and buffer salts from 0.1 mL of 0.01 M sodium phosphate, pH 7.5.
Reconstitution
Reconstitute with 0.1 mL water
Unit Definition
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Sigma Aldrich -  C9972 external link
Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
Quality
Biotin content ~1.0 mole/mole protein.
Physical form
Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Sigma Aldrich -  C167 external link
Caution
Do not freeze.
General description
Low salt
Reconstitution
When reconstituted in 0.25 mL distilled water, the compound contains 500 μg of protein in 0.01 M sodium phosphate at pH 7.5.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Sigma Aldrich -  C7771 external link
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Sigma Aldrich -  C1655 external link
Analysis Note
Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

参考文献

参考文献

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专利

专利

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