2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2$l^{5},3,4-tetrazol-2-ylium bromide

• ChemBase编号: 102580
• 分子式: C18H16BrN5S
• 平均质量: 414.32214
• 单一同位素质量: 413.03097854
• SMILES: [Br-].Cc1c(C)nc(s1)[n+]1nc(nn1c1ccccc1)c1ccccc1
• Canonical SMILES: Cc1sc(nc1C)[n+]1nc(nn1c1ccccc1)c1ccccc1.[Br-]
• InChI: InChI=1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChIKey: AZKSAVLVSZKNRD-UHFFFAOYSA-M

名称和登记号

名称

• IUPAC标准名: 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2$l^{5},3,4-tetrazol-2-ylium bromide; 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ^5,3,4-tetrazol-2-ylium bromide
• IUPAC传统名: 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-1,2$l^{5},3,4-tetrazol-2-ylium bromide; 2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-1,2λ^5,3,4-tetrazol-2-ylium bromide
• 别名: 噻唑蓝溴化四唑; MTT; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide; 3-(4,5-DIMETHYLTHIAZOLYL-2)-2,5-DIPHENYL TETRAZOLIUM BROMIDE; Thiazolyl Blue; 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide; Thiazolyl Blue Tetrazolium Bromide

登记号

• CAS号: 298-93-1
• EC号: 206-069-5
• MDL号: MFCD00011964
• Beilstein号: 3825277; 4081397
• PubChem SID: 24889092; 24896603; 162090196; 24897030; 24848194
• PubChem CID: 64965

理论计算性质

• 质子受体: 3
• 质子供体: 0
• LogD (pH = 5.5): 2.741026
• LogD (pH = 7.4): 2.741026
• Log P: 2.741026
• 摩尔折射率: 128.6325 cm^3
• 极化性: 37.12812 Å^3
• 极化表面积: 47.48 Å^2
• 可自由旋转的化学键: 3
• 里宾斯基五规则: true

分子性质

理化性质

• 溶解度: H2O: soluble5 mg/mL; methanol: soluble5 mg/mL, clear
• 外观: powder
• 熔点: 195 °C (dec.)(lit.); 195°C; ca 195°C dec.
• 紫外吸收波长: λmax 378 nm

安全信息

• 保存条件: 2-8°C, Protect from light
• 保存注意事项: Light Sensitive
• RTECS编号: XF8060000
• 欧盟危险性物质标志: 刺激性(Irritant) (Xi); 有害性(Harmful) (Xn)
• 德国WGK号: 3
• 危险公开号: 36/37/38; 36/37/38-68
• 安全公开号: 26; 26-36/37; 26-37-60
• TSCA收录: 是
• GHS危险品标识: GHS07; GHS08
• GHS警示词: Warning
• GHS危险声明: H315-H319-H335-H341
• GHS警示性声明: P261-P281-P305 + P351 + P338
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 保存温度: 2-8°C

产品相关信息

• 纯度: ~98%; ≥97.0% (enzymatic); ≥97.5% (HPLC); 98%
• 适用性: suitable for cell culture; suitable for insect cell culture
• 产品线: BioReagent
• Empirical Formula (Hill Notation): C18H16BrN5S

详细说明

MP Biomedicals - 02194592

Cell Culture Reagent
Yellow crystals
Purity: ~98%

MP Biomedicals - 02102227

Yellow crystals
Purity: ~98%

Sigma Aldrich - M5655

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
包装
1 g in glass bottle
15, 100, 500 mg in glass bottle

Sigma Aldrich - M2128

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
包装
1, 5, 10 g in glass bottle
100, 250, 500 mg in glass bottle
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9

Sigma Aldrich - 135038

包装
1 g in glass bottle
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9

Sigma Aldrich - 88415

Other Notes
Review: Tetrazolium salts and formazans10
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9