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MG132

产品号 S2619 公司名称 Selleck Chemicals
CAS号 133407-82-6 公司网站 http://www.selleckchem.com
分子式 C26H41N3O5 电 话 (877) 796-6397
分子量 475.62084 传 真 (832) 582-8590
纯 度 电子邮件 sales@selleckchem.com
保 存 -20°C Chembase数据库ID: 73098

产品价格信息

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产品别名

标题
MG132
IUPAC标准名
benzyl N-[(1S)-3-methyl-1-{[(1S)-3-methyl-1-{[(2S)-4-methyl-1-oxopentan-2-yl]carbamoyl}butyl]carbamoyl}butyl]carbamate
IUPAC传统名
benzyl N-[(1S)-3-methyl-1-{[(1S)-3-methyl-1-{[(2S)-4-methyl-1-oxopentan-2-yl]carbamoyl}butyl]carbamoyl}butyl]carbamate
别名
MG-132

产品登记号

CAS号 133407-82-6

产品性质

作用靶点 Proteasome
成盐信息 Free Base
保存条件 -20°C

产品详细信息

详细说明 (English)
Research Area
Description Cancer
Protocol
Kinase Assay [1]
Measurement of inhibitory activities of MG-132 against 20S proteasome The reaction mixture for the 20S proteasome inhibitory assay contains 0.1 M Tris-acetate, pH 7.0, 20S proteasome, MG-132, and 25 μM substrate dissolved in dimethyl sulfoxide in a final volume of 1 mL. After incuba tion at 37 °C for 15 minutes, the reaction is stopped by the addition of 0.1 mL of 10% SDS and 0.9 mL of 0.1M Tris acetate, pH 9.0. The fluorescence of the reaction products is measured. To determine the IC50 against 20S proteasome, various concentrations of MG-132 are included in the assay mixture.
Cell Assay [5]
Cell Lines KIM-2, HC11, and ES
Concentrations Dissolved in DMSO, final concentrations ~25 μM
Incubation Time 24, and 48 hours
Methods Cells are exposed to various concentrations of MG-132 for 24, and 48 hours. Supernatant and monolayer cells are harvested by centrifugation and fixed in 70% ethanol in PBS for staining with acridine orange. Equal volumes of cells and acridine orange (5 mg/mL in PBS) are mixed on a microscope slide and examined by fluorescence microscopy. For annexin V analysis, cells are harvested by centrifugation and stained with annexin V and propidium iodide. For cell cycle analysis, cells are rehydrated in PBS at room temperature for 10 minutes, followed by staining with propidium iodide (5 mg/mL). All samples are analyzed using a Coulter Epics XL flow cytometer.
Animal Study [6]
Animal Models Male mdx (C57BL/10ScSn DMD mdx) mice
Formulation Dissolved in DMSO, and diluted in PBS
Doses ~10 μg/kg/day
Administration Injection
References
[1] Tsubuki S, et al. J Biochem, 1996, 119(3), 572-576.
[2] Fiedler MA, et al. Am J Respir Cell Mol Biol, 1998, 19(2), 259-268.
[3] Giuliano M, et al. Cancer Res, 1999, 59(21), 5586-5595.
[4] Arlt A, et al. Oncogene, 2001, 20(7), 859-868.
[5] MacLaren AP, et al. Cell Death Differ, 2001, 8(3), 210-218.
[6] Bonuccelli G, et al. Am J Pathol, 2003, 163(4), 1663-1675.