PTC124

• 产品号: S6003
• CAS号: 775304-57-9
• 分子式: C15H9FN2O3
• 分子量: 284.2419632
• 保 存: -20°C

产品别名

• 标题: PTC124
• IUPAC标准名: 3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
• IUPAC传统名: ataluren
• 别名: Ataluren

产品登记号

• CAS号: 775304-57-9

产品性质

• 成盐信息: Free Base
• 溶解度: DMSO
• 保存条件: -20°C

产品详细信息

详细说明 (English)

Research Area

• Description: Inflammation

Protocol

• Protocol: Kinase Assay ^[1]
• Assays for read-through of LUC premature termination codons: Stock solutions of PTC124 (6 mM) are prepared in 100% DMSO HEK293 cells grown in a medium containing fetal bovine serum (FBS) and are stably transfected with LUC genes containing premature terminators at codon 190 (and 11 contextual variants thereof), treated with increasing concentrations of PTC124 for 16 hours, and assayed for luciferase activity. Luminescence of each culture relative to the control is quantified by standard procedures using a Viewlux CCD imager. Luminescence data are normalized to that produced with DMSO, and the fold suppression over background is calculated as (PTC124_{light units}/DMSO_{light units}).
• Protocol: Animal Study ^[2]
• Animal Models: Cftr-/- hCFTR-G542X transgenic mice
• Formulation: Dissolved in DMSO, and diluted in saline
• Doses: ~60 mg/kg/day
• Administration: Subcutaneous injection or oral administration

References

• References: [1] Welch EM, et al. Nature, 2007, 447(7140), 87-91.
• References: [2] Du M, et al. Proc Natl Acad Sci U S A, 2008, 105(6), 2064-2069.

参考文献

• Welch EM, et al. Nature, 2007, 447(7140), 87-91.
• Du M, et al. Proc Natl Acad Sci U S A, 2008, 105(6), 2064-2069.