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溴化乙啶 溶液_分子结构_CAS_1239-45-8)
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溴化乙啶 溶液

产品号 09-0617 公司名称 Sigma Aldrich
CAS号 1239-45-8 公司网站 http://www.sigmaaldrich.com
分子式 C21H20BrN3 电 话 1-800-521-8956
分子量 394.3076 传 真
纯 度 电子邮件
保 存 Chembase数据库ID: 127319

产品价格信息

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产品别名

标题
Ethidium bromide solution
IUPAC标准名
3,8-diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide
IUPAC传统名
ethidium bromide
别名
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Homidium bromide
EtBr
3,8-二氨基-5-乙基-6-苯基菲啶溴化物
胡米溴铵

产品登记号

Beilstein号 3642536
CAS号 1239-45-8
MDL号 MFCD00011724

产品性质

浓度 10 mg/mL
Empirical Formula (Hill Notation) C21H20BrN3
欧盟危险性物质标志 有毒(Toxic) 有毒(Toxic) (T)
RID/ADR UN 2810 6.1/PG 3
危险公开号 23-68
RTECS编号 SF7950000
安全公开号 36/37-45
联合国危险货物等级 6.1
联合国危险货物编号 2810
联合国危险货物包装类别(PG) 3
德国WGK号 2

产品详细信息

详细说明 (English)
Application
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.1,2,3 It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.5
Biochem/physiol Actions
Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Reconstitution
For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.
Suitability
for biological purposes
详细说明 (简体中文)
Application
溴化乙啶 (EtBr) 为聚丙烯酰胺凝胶电泳或琼脂糖凝胶电泳中最常用的核酸染料。EtBr 在结合到双链 RNA 上时其荧光可提高 21 倍,在结合到双链 DNA 上可提高 25 倍,从而使得在低染料浓度 (10μg/ml) 下无需背景脱色。溴化乙啶已用于核酸的许多荧光分析。1,2,3已表明它可结合到单链 DNA(虽然强度不高)和三链 DNA 上。4由于 EtBr 能够结合到 DNA 上,因此它是 DNA 聚合酶的抑制剂。5
Biochem/physiol Actions
溴化乙啶可插入双链 DNA 和 RNA 中,并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。
Reconstitution
对于在电泳后进行凝胶染色,应先用水将储存液样品稀释到 0.5μg/ml,再将凝胶孵育 15-30 分钟。通常无需脱色,但是如果必须降低背景色,则可在水中脱色 15 分钟。然后可在紫外灯箱(254nm 波长)中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液(终浓度 0.5μg/ml)中,并在电泳后立即显影。
Suitability
for biological purposes

参考文献