CHAPS

• 产品号: 26680
• CAS号: 75621-03-3
• 分子式: C32H58N2O7S
• 分子量: 614.87712
• 纯 度: ≥98.0% (TLC)

产品别名

• 标题: CHAPS
• IUPAC标准名: 3-[dimethyl({3-[(4R)-4-[(1S,2S,5R,9R,10R,11S,14R,15R,16S)-5,9,16-trihydroxy-2,15-dimethyltetracyclo[8.7.0.0^2,^7.0^1^1,^1^5]heptadecan-14-yl]pentanamido]propyl})azaniumyl]propane-1-sulfonate
• IUPAC传统名: 3-[dimethyl({3-[(4R)-4-[(1S,2S,5R,9R,10R,11S,14R,15R,16S)-5,9,16-trihydroxy-2,15-dimethyltetracyclo[8.7.0.0^2,^7.0^1^1,^1^5]heptadecan-14-yl]pentanamido]propyl})ammonio]propane-1-sulfonate
• 别名: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate; 3-[(3-胆酰胺丙基)二甲氨基]丙磺酸内盐

产品登记号

• MDL号: MFCD00012116
• CAS号: 75621-03-3

产品性质

• 痕量阴离子: chloride (Cl-): ≤50 mg/kg
• 痕量阴离子: sulfate (SO42-): ≤50 mg/kg
• 痕量阳离子: Ca: ≤10 mg/kg
• 痕量阳离子: Cd: ≤5 mg/kg
• 痕量阳离子: Co: ≤5 mg/kg
• 痕量阳离子: Cr: ≤5 mg/kg
• 痕量阳离子: Cu: ≤5 mg/kg
• 痕量阳离子: Fe: ≤5 mg/kg
• 痕量阳离子: K: ≤50 mg/kg
• 痕量阳离子: Mg: ≤5 mg/kg
• 痕量阳离子: Mn: ≤5 mg/kg
• 痕量阳离子: Na: ≤50 mg/kg
• 痕量阳离子: Ni: ≤5 mg/kg
• 痕量阳离子: Pb: ≤5 mg/kg
• 痕量阳离子: Zn: ≤5 mg/kg
• 简要说明: zwitterionic
• Empirical Formula (Hill Notation): C32H58N2O7S
• 杂质: ≤8% water
• Mol. Weight: micellar avg mol wt 6150
• 纯度: ≥98.0% (TLC)
• 胶束聚集数: 10
• 临界胶束浓度: 6 mM(20-25°C)
• 溶解度: H2O: soluble50 mg/mL at 20 °C, clear, colorless
• 转变温度: cloud point >100 °C
• 欧盟危险性物质标志: 有毒(Toxic) (T)
• 危险公开号: 61
• 安全公开号: 53-45
• 德国WGK号: -

产品详细信息

详细说明 (English)

Application
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Other Notes
Zwitterionic detergent for the solubilisation of membrane proteins. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents1,2; As a non-toxic stabilizing agent for growth factors3

详细说明 (简体中文)

Application
CHAPS 是一种用于膜生物化学的非变性两性离子洗涤剂。 可用于溶解膜蛋白以及打断蛋白-蛋白相互作用。CHAPS 具有低胶束分子量 (6,150) 和高临界胶束浓度 (6-10mM)，因此可通过透析从样品中除去。它也适合在等电聚焦和二维电泳中用于溶解蛋白质。CHAPS 常用于非变性（无尿素）等电聚焦，已发现它对某些亚细胞制备物和植物蛋白能够得出优异的分辨率。通常将介于 2-4% (w/v) 间的浓度用于等电聚焦凝胶。
Other Notes
用于溶解膜蛋白的两性离子型洗涤剂。结合了硫代甜菜碱型洗涤剂和胆盐洗涤剂的有用性质1,2；作为生长因子的无毒性稳定剂3