Fluorescent red 635 reactive

• 产品号: 39779
• CAS号: 350496-77-4
• 分子式: C42H49N3O8S
• 分子量: 755.91876

产品别名

• 标题: Fluorescent red 635 reactive
• IUPAC标准名: 2-(3-{4-tert-butyl-3-oxa-13-azatetracyclo[7.7.1.0^2,^7.0^1^3,^1^7]heptadeca-1(17),2(7),4,8-tetraen-6-ylidene}prop-1-en-1-yl)-1-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
• IUPAC传统名: 2-(3-{4-tert-butyl-3-oxa-13-azatetracyclo[7.7.1.0^2,^7.0^1^3,^1^7]heptadeca-1(17),2(7),4,8-tetraen-6-ylidene}prop-1-en-1-yl)-1-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-3,3-dimethylindol-1-ium-5-sulfonate
• 别名: 2-[3-(11-tert.-Butylpyrano[3,2-g]pyrido[3,2,1-ij]quinolin-9-ylidene)-1-propenyl]-1-[5-(succinimidooxycarbonyl)-pentyl]-3,3-dimethylindolio-5-sulfonate

产品登记号

• CAS号: 350496-77-4
• MDL号: MFCD03099336
• PubChem SID: 24864707

产品性质

• Empirical Formula (Hill Notation): C42H49N3O8S
• 级别: for fluorescence
• 荧光: λex 635 nm; λem 665 nm in 0.1 M phosphate pH 7.0 (non-covalent binding to BSA enhances fluorescence emission)
• 溶解度: DMF: soluble
• GHS警示词: Warning
• GHS危险声明: H315-H319-H335
• 欧盟危险性物质标志: 刺激性(Irritant) (Xi)
• 个人保护装置: dust mask type N95 (US), Eyeshields, Gloves
• GHS警示性声明: P261-P305 + P351 + P338
• 危险公开号: 36/37/38
• 安全公开号: 26
• 保存温度: 2-8°C
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Application
Amino-reactive biolabel excited by red lasers (He-Ne; 633 nm) and diode lasers (635-650 nm). Molar absorption 200′000 1/M cm, abs: (conjugate) 650 nm, em: (conjugate) 670 nm, QY is fairly low in aqueous media, but up to 0.4 for conjugates.
Features and Benefits
Hemicyanine dye Fluorescent red 630 match excitation by He-Ne laser line at 633 nm, Argon or Argon-Krypton laser lines at 647 nm and diode lasers (635-650 nm). BenefitsIdeal match to common excitation light sourcesHigh molar absorption (200.000 M-1cm-1) Fairly low quantum yield if dissolved inaqueous media, but very strong increase by coupling to target molecule.Superior solid state fluorescence making them certainly useful to label DNA for preparation of glass arrays
General description
Fluorescent red NHS is an aminoreactive fluorescent biolabel designed for excitation by red lasers (He/Ne; 633 nm) and diode lasers (635 - 650 nm). It can be used for covalent coupling to proteins and other biomolecules containing primary amino groups like aminomodified DNA-oligomers and aminomodified biotin. The chromophore responsible for absoprtion maximum is based on a bridged hemicyanine.Fluorescent red 635-NHS exhibits a fairly low fluorescence quantum yield if dissolved in aqueous media, but after coupling to a target signal intensity can rise up to one order of magnitude as demonstrated for protein conjugates and labeled oligomers. Together with the quantum yield the fluorescence lifetime increases drastically.Within the conjugate absorption of the label shifts slightly to the red while position and shape of the emission spectrum remain nearly unchanged.

详细说明 (简体中文)

Application
Amino-reactive biolabel excited by red lasers (He-Ne; 633 nm) and diode lasers (635-650 nm). Molar absorption 200′000 1/M cm, abs: (conjugate) 650 nm, em: (conjugate) 670 nm, QY is fairly low in aqueous media, but up to 0.4 for conjugates.
Features and Benefits
Hemicyanine dye Fluorescent red 630 match excitation by He-Ne laser line at 633 nm, Argon or Argon-Krypton laser lines at 647 nm and diode lasers (635-650 nm). BenefitsIdeal match to common excitation light sourcesHigh molar absorption (200.000 M-1cm-1) Fairly low quantum yield if dissolved inaqueous media, but very strong increase by coupling to target molecule.Superior solid state fluorescence making them certainly useful to label DNA for preparation of glass arrays
General description
Fluorescent red NHS is an aminoreactive fluorescent biolabel designed for excitation by red lasers (He/Ne; 633 nm) and diode lasers (635 - 650 nm). It can be used for covalent coupling to proteins and other biomolecules containing primary amino groups like aminomodified DNA-oligomers and aminomodified biotin. The chromophore responsible for absoprtion maximum is based on a bridged hemicyanine.Fluorescent red 635-NHS exhibits a fairly low fluorescence quantum yield if dissolved in aqueous media, but after coupling to a target signal intensity can rise up to one order of magnitude as demonstrated for protein conjugates and labeled oligomers. Together with the quantum yield the fluorescence lifetime increases drastically.Within the conjugate absorption of the label shifts slightly to the red while position and shape of the emission spectrum remain nearly unchanged.