Application 邻苯二胺是一种适用于 ELISA 分析的过氧化物酶底物。它可生成黄褐色的可溶性终产物,而该产物可通过分光光度法在 450nm 处读取。可用 3N HCl 或 3M H2SO4 中止 OPD 反应,并在 492nm 处读取。 Reconstitution Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets. |