邻苯二胺 二盐酸盐

• 产品号: P8287
• CAS号: 615-28-1
• 分子式: C6H10Cl2N2
• 分子量: 181.063

产品别名

• 标题: o-Phenylenediamine dihydrochloride
• IUPAC标准名: benzene-1,2-diamine dihydrochloride
• IUPAC传统名: o-phenylenediamine dihydrochloride

产品登记号

• PubChem SID: 24898741
• CAS号: 615-28-1
• Beilstein号: 3912045
• MDL号: MFCD00012966

产品性质

• 线性分子式: C6H4(NH2)2·2HCl
• 产品质量级别: PREMIUM
• 外观: tablet
• 熔点: 258 °C (dec.)(lit.)
• GHS警示词: Warning
• GHS危险声明: H302-H317-H319-H332-H341-H351-H410
• 欧盟危险性物质标志: 有毒(Toxic) (T)
• 欧盟危险性物质标志: 环境危害性(Nature polluting) (N)
• GHS警示性声明: P273-P280-P305 + P351 + P338-P501
• RID/ADR: UN 1673 6.1/PG 3
• 危险公开号: 20/21-25-36-40-43-50/53-68
• 安全公开号: 26-36/37-45-61
• 保存温度: 2-8°C
• 联合国危险货物等级: 6.1
• 联合国危险货物编号: 1673
• 联合国危险货物包装类别(PG): 3
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Application
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

详细说明 (简体中文)

Application
邻苯二胺是一种适用于 ELISA 分析的过氧化物酶底物。它可生成黄褐色的可溶性终产物，而该产物可通过分光光度法在 450nm 处读取。可用 3N HCl 或 3M H2SO4 中止 OPD 反应，并在 492nm 处读取。
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.