DL-二硫苏糖醇

• 产品号: 43817
• CAS号: 3483-12-3
• 分子式: C4H10O2S2
• 分子量: 154.251
• 纯 度: ≥99.0% (RT)

产品别名

• 标题: DL-Dithiothreitol
• IUPAC标准名: (2S,3S)-1,4-disulfanylbutane-2,3-diol
• IUPAC传统名: dithiothreitol
• 别名: threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT; 1,4-二巯基苏糖醇; 克莱兰氏试剂

产品登记号

• MDL号: MFCD00004877
• EC号: 222-468-7
• CAS号: 3483-12-3
• PubChem SID: 24867429
• Beilstein号: 1719757

产品性质

• 痕量阴离子: sulfate (SO42-): ≤50 mg/kg
• 痕量阳离子: Al: ≤5 mg/kg
• 痕量阳离子: As: ≤0.5 mg/kg
• 痕量阳离子: Ba: ≤5 mg/kg
• 痕量阳离子: Bi: ≤5 mg/kg
• 痕量阳离子: Ca: ≤10 mg/kg
• 痕量阳离子: Cd: ≤5 mg/kg
• 痕量阳离子: Co: ≤5 mg/kg
• 痕量阳离子: Cr: ≤5 mg/kg
• 痕量阳离子: Cu: ≤5 mg/kg
• 痕量阳离子: Fe: ≤5 mg/kg
• 痕量阳离子: K: ≤50 mg/kg
• 痕量阳离子: Li: ≤5 mg/kg
• 痕量阳离子: Mg: ≤5 mg/kg
• 痕量阳离子: Mn: ≤5 mg/kg
• 痕量阳离子: Mo: ≤5 mg/kg
• 痕量阳离子: Na: ≤100 mg/kg
• 痕量阳离子: Ni: ≤5 mg/kg
• 痕量阳离子: Pb: ≤5 mg/kg
• 痕量阳离子: Sr: ≤5 mg/kg
• 痕量阳离子: Zn: ≤5 mg/kg
• 杂质: insoluble matter, passes filter test
• 杂质: ≤0.3% 4,5-dihydroxy-1,2-dithiane
• λ: 0.1 M in H2O
• 线性分子式: HSCH2CH(OH)CH(OH)CH2SH
• 产品线: BioUltra
• 纯度: ≥99.0% (RT)
• 紫外吸收波长: λ: 260 nm Amax: 0.400
• 紫外吸收波长: λ: 280 nm Amax: 0.100
• 闪点: 113 °C
• 闪点: 235.4 °F
• 熔点: 41-44 °C(lit.)
• pH值: 4.0-6.5 (25 °C, 0.1 M in H2O)
• 溶解度: H2O: soluble0.1 M at 20 °C, clear, colorless
• GHS警示词: Warning
• GHS危险声明: H302-H315-H319-H335
• 欧盟危险性物质标志: 有害性(Harmful) (Xn)
• 个人保护装置: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• GHS警示性声明: P261-P305 + P351 + P338
• 危险公开号: 22-36/37/38
• RTECS编号: EK1610000
• 安全公开号: 26-36
• 保存温度: 2-8°C
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Other Notes
Cleavage of disulfide bonds in proteins;1 In the investigation of incremental and kinetic reduction in proteins;2 Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues;3 In protein sequence analysis.4

详细说明 (简体中文)

Application
用于将 -SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。
Other Notes
Cleavage of disulfide bonds in proteins;1 In the investigation of incremental and kinetic reduction in proteins;2 Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues;3 In protein sequence analysis.4