Atto 425 NHS ester

• 产品号: 16805
• CAS号: 892156-28-4
• 分子式: C26H30N2O8
• 分子量: 498.525

产品别名

• 标题: Atto 425 NHS ester
• IUPAC标准名: ethyl 9-{4-[(2,5-dioxopyrrolidin-1-yl)oxy]-4-oxobutyl}-6,8,8-trimethyl-2-oxo-2H,6H,7H,8H,9H-chromeno[7,6-b]pyridine-3-carboxylate
• IUPAC传统名: atto 425 nhs-ester
• 别名: Atto 425-N-hydroxysuccinimide ester

产品登记号

• MDL号: MFCD04974819
• CAS号: 892156-28-4
• PubChem SID: 24850231

产品性质

• Empirical Formula (Hill Notation): C26H30N2O8
• 产品线: BioReagent
• 适用性: suitable for fluorescence
• 荧光: λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
• 溶解度: DMF: soluble
• 溶解度: DMSO: soluble
• 溶解度: H2O: soluble
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 保存温度: -20°C
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components 1. Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria 2. When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells 3. Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation 4.
Other Notes
Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS5; employed in triple-color coincidence analysis6.

详细说明 (简体中文)

Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components 1. Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria 2. When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells 3. Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation 4.
Other Notes
Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS5; employed in triple-color coincidence analysis6.