Puromycin

• 产品号: P9620
• CAS号: 58-58-2
• 分子式: C22H31Cl2N7O5
• 分子量: 544.43144
• 纯 度: >98% (HPLC)

产品别名

• 标题: Puromycin dihydrochloride
• IUPAC标准名: (2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)-9H-purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide dihydrochloride
• IUPAC传统名: puromycin dihydrochloride

产品登记号

• MDL号: MFCD00012691
• CAS号: 58-58-2

产品性质

• 生物来源: from Streptomyces alboniger
• 浓度: 10 mg/mL in H2O
• 纯度: >98% (HPLC)
• 产品质量级别: PREMIUM
• 运输包装: wet ice
• 无菌消毒: 0.2 μm filtered
• 适用性: cell culture tested
• 个人保护装置: Eyeshields, Faceshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
• 保存温度: -20°C
• 德国WGK号: 2

产品详细信息

详细说明 (English)

Application
Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model 1. Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer2.
Biochem/physiol Actions
Puromycin inhibits bacterial and mammalian protein synthesis by causing premature chain termination during translation taking place in the ribosome. Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
Suitability
Tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

详细说明 (简体中文)

Application
Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model 1. Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer2.
Biochem/physiol Actions
Puromycin inhibits bacterial and mammalian protein synthesis by causing premature chain termination during translation taking place in the ribosome. Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
Suitability
Tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information