Application Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. General description Atto 465 is a new label with strong absorption and fluorescence and a large stoke′s shift (excitation maximum 453 nm, emission maximum 508 nm). The dye is characterized by high photostability and thermal stability. The dye is moderately hydrophilic, the NHS-ester and maleimide derivatives are very well soluble in polar solvents as DMSO, DMF or acetonitrile. At low temperatures or embedded into polymeric matrices it shows intense phosphorescence. |