G 418 disulfate salt solution

• 产品号: G8168
• CAS号: 108321-42-2
• 分子式: C20H44N4O18S2
• 分子量: 692.70936

产品别名

• 标题: G 418 disulfate salt solution
• IUPAC标准名: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• IUPAC传统名: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• 别名: Antibiotic G418

产品登记号

• CAS号: 108321-42-2
• MDL号: MFCD00058314
• PubChem SID: 24895333

产品性质

• 浓度: 50 mg/mL in H2O
• Empirical Formula (Hill Notation): C20H40N4O10 · 2H2SO4
• 杂质: endotoxin, tested
• 产品线: BioReagent
• 无菌消毒: sterile-filtered
• 适用性: suitable for cell culture
• GHS警示词: Danger
• GHS危险声明: H317-H334
• 欧盟危险性物质标志: 有害性(Harmful) (Xn)
• GHS警示性声明: P261-P280-P342 + P311
• 危险公开号: 42/43
• 安全公开号: 23-36/37-45
• 保存温度: 2-8°C
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Biochem/physiol Actions
Mode of Action: Blocks polypeptide synthesis and inhibits elongation in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene
Physical form
50 mg/mL in tissue culture grade water
Application
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

详细说明 (简体中文)

Biochem/physiol Actions
Mode of Action: Blocks polypeptide synthesis and inhibits elongation in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene
Physical form
50 mg/mL in tissue culture grade water
Application
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information