G 418 二硫酸盐

• 产品号: G5013
• CAS号: 108321-42-2
• 分子式: C20H44N4O18S2
• 分子量: 692.70936

产品别名

• 标题: G 418 disulfate salt
• IUPAC标准名: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• IUPAC传统名: (2R,3S,4R,5R,6S)-5-amino-6-{[(1R,2S,3S,4R,6S)-4,6-diamino-3-{[(2S,3S,4S,5S)-3,5-dihydroxy-5-methyl-4-(methylamino)oxan-2-yl]oxy}-2-hydroxycyclohexyl]oxy}-2-(1-hydroxyethyl)oxane-3,4-diol; bis(sulfuric acid)
• 别名: 抗生物質G418

产品登记号

• CAS号: 108321-42-2
• MDL号: MFCD00058314
• PubChem SID: 24895193

产品性质

• 药效: ≥450 μg per mg (dried basis)
• 外观: white powder
• 溶解度: H2O: soluble50 mg/mL
• GHS警示词: Danger
• GHS危险声明: H317-H334
• 欧盟危险性物质标志: 有害性(Harmful) (Xn)
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• GHS警示性声明: P261-P280-P342 + P311
• 危险公开号: 42/43
• 安全公开号: 22-36/37-45
• 保存温度: 2-8°C
• 德国WGK号: 3

产品详细信息

详细说明 (English)

Biochem/physiol Actions
G 418 blocks polypeptide synthesis by interfering with ribosomal function and inhibiting protein elongation.
Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes.
Caution
Stable at 37 °C for 8 days.
Other Notes
Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin.
Reconstitution
Aqueous solutions stored frozen at -20 to -70 °C should be stable for approximately six months.
Application
Blocks protein synthesis by interfering with ribosomal function. Not normally used as a standard antibiotic, but rather for selection and maintenance of prokaryotic and eukaryotic cells stably transfected with neomycin resistance genes. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. The resistance genes are APH (3′) II or aminoglycoside phosphotransferase 3′(II) and aminoglycoside phosphotranferase 3′ (I) or APH (3′) I. The following concentrations of active ingredient may be used as a guide for selection: mammalian cells (400 ug/ml), plants (10 ug/ml), yeasts (500 ug/ml), and dictyostelium (10 ug/ml). Cells have variable resistance and multiplying cells will be affected sooner than those that are not. Cells in log phase may require three to seven days for selection. Varying concentrations should be tested. Additionally, fibroblast contamination in mixed cultures may be suppressed by using 100 ug/ml of the active ingredient for 2 days.
G 418 is a Micromonospora-produced aminoglycoside antibiotic with activity against protozoa and helminths. G 418 disulfate salt is used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information