Ac-Asp-Glu-Val-Asp-paranitroanilide

• 产品号: 03PNA138
• CAS号: 189950-66-1
• 分子式: C26H34N6O13
• 分子量: 638.58056

产品别名

• 标题: Ac-Asp-Glu-Val-Asp-paranitroanilide
• IUPAC标准名: (4S)-4-{[(1S)-1-{[(1S)-2-carboxy-1-[(4-nitrophenyl)carbamoyl]ethyl]carbamoyl}-2-methylpropyl]carbamoyl}-4-[(2S)-3-carboxy-2-acetamidopropanamido]butanoic acid
• IUPAC传统名: (4S)-4-{[(1S)-1-{[(1S)-2-carboxy-1-[(4-nitrophenyl)carbamoyl]ethyl]carbamoyl}-2-methylpropyl]carbamoyl}-4-[(2S)-3-carboxy-2-acetamidopropanamido]butanoic acid
• 别名: Ac-D-E-V-D-PNA; Ac-Asp-Glu-Val-Asp-PNA

产品登记号

• CAS号: 189950-66-1

产品详细信息

详细说明 (English)

A colorimetric substrate for caspase-3 (Km = 9.7 μM), CCP-32 and related cysteine proteases. Sequence is based on the P1-P4 tetrapeptide cleavage site of poly(ADP-ribose) polymerase (PARP) and includes Asp216. Caspase-3 is the protease responsible for the cleavage of PARP. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10. Cleavage of pNA is monitored colorimetrically at ~405 nm.

参考文献

• Thornberry, N.A. et al., Science, 281:1312 (1998).
• Nicholson, D.W. et al., Nature, 376:37 (1995).
• Cardone, M.H., et al. 1998. Science 282, 1318.
• Lazebnik, Y.A., et al. 1994. Nature 371, 346.