4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline

• ChemBase编号: 74451
• 分子式: C16H20N2
• 平均质量: 240.3434
• 单一同位素质量: 240.16264865
• SMILES: Nc1c(cc(cc1C)c1cc(c(c(c1)C)N)C)C
• Canonical SMILES: Cc1cc(cc(c1N)C)c1cc(C)c(c(c1)C)N
• InChI: InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChIKey: UAIUNKRWKOVEES-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
• IUPAC传统名: tetramethylbenzidine
• 别名: 3,3′,5,5′-四甲基联苯胺; 3,3',5,5'-四甲基联苯胺; 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine; 3,3',5,5'-Tetramethyl benzidine; 3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA; TMB; 3,3',5,5'-TETRAMETHYLBENZIDINE LIQUID SUBSTRATE; BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; 3,3′,5,5′-Tetramethylbenzidine; 3,3',5,5'-Tetramethylbenzidine; 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl; 3,3',5,5'-Tetramethylbenzidine

登记号

• CAS号: 54827-17-7
• EC号: 259-364-6
• MDL号: MFCD00007748
• Beilstein号: 2808541
• PubChem SID: 24900293; 162039370; 24846282; 24900209; 24888505; 24900090
• PubChem CID: 41206
• Chemspider ID: 37605
• 维基百科标题: 3,3',5,5'-Tetramethylbenzidine

理论计算性质

• 质子受体: 2
• 质子供体: 2
• LogD (pH = 5.5): 3.980853
• LogD (pH = 7.4): 4.015844
• Log P: 4.016305
• 摩尔折射率: 80.7598 cm^3
• 极化性: 30.724943 Å^3
• 极化表面积: 52.04 Å^2
• 可自由旋转的化学键: 1
• 里宾斯基五规则: true

分子性质

理化性质

• 外观: tablet
• 熔点: 166-171 °C; 168-169°C; 168-171 °C; 168-171 °C(lit.); 168-171°C; 168-171°C

安全信息

• 保存条件: 2-8°C, Protect from light; Room Temperature (15-30°C), Protect from light
• 保存注意事项: Irritant/Light Sensitive/Store under Argon; Light Sensitive
• RTECS编号: DV2300000
• 欧盟危险性物质标志: X; 刺激性(Irritant) (Xi)
• 德国WGK号: 3
• 危险公开号: 22-36/37/38; 36; 36/37/38
• 安全公开号: 26; 26-36; 26-36/37
• TSCA收录: 是
• GHS危险品标识: GHS06; GHS07
• GHS警示词: Warning
• GHS危险声明: H301-H315-H319-H335; H315-H319-H335; H319
• GHS警示性声明: P261-P301+P310-P305+P351+P338-P302+P352-P405-P501A; P261-P305 + P351 + P338; P305 + P351 + P338
• 个人保护装置: dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• 保存温度: 2-8°C

产品相关信息

• 纯度: ≥99%; ≥98% (TLC); ≥99%; ≥99.0% (GC); 98%
• 级别: puriss. p.a.; standard for GC
• 有效期: (limited shelf life, expiry date on the label)
• 运输包装: wet ice
• 线性分子式: [-C6H2(CH3)2-4-NH2]2

详细说明

MP Biomedicals - 02152346

Ready-to-Use • Convenient • Reproducible Results • Consistent Performance and Sensitivity Stabilized chromogen solution, 1.25 mmol/liter, for ELISA procedures and peroxidase and pseudo-peroxidase reactions. Stable up to 24 months. Non toxic.

MP Biomedicals - 02190277

Crystalline
Purity: 99+%
Reported to be a noncarcinogenic^1 analog of benzidine; useful in gel staining procedure for low levels of heme-associated peroxidases^2 and for enzyme immunoassay of horseradish peroxidase^3 .

Sigma Aldrich - T5525

Application
本品为联苯胺1的非致癌性替代品(Ames 试验阴性)，适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色)，并在 450nm 处读取数据。一种灵敏的特异性试剂，用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8

Sigma Aldrich - T4444

Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Sigma Aldrich - T2885

Application
本品为联苯胺1的非致癌性替代品(Ames 试验阴性)，适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色)，并在 450nm 处读取数据。一种灵敏的特异性试剂，用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8

Sigma Aldrich - 860336

Application
本品为联苯胺1的非致癌性替代品(Ames 试验阴性)，适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色)，并在 450nm 处读取数据。一种灵敏的特异性试剂，用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8
包装
1, 5, 25 g in glass bottle
100 mg in glass bottle

Sigma Aldrich - 09743

Application
本品为联苯胺1的非致癌性替代品(Ames 试验阴性)，适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色)，并在 450nm 处读取数据。一种灵敏的特异性试剂，用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8
Other Notes
用于测定商品中偶氮染料所释放的芳香胺的标准品9

参考文献

• Holland, V.R., et al., Tetrahedron , 30 : 3299, (1976).
• Anal. Biochem. , 75 : 168, (1976).
• Bos, E.E., et al., J. Immunoassay , 2 : 187, (1983).