2-amino-2-(hydroxymethyl)propane-1,3-diol

• ChemBase编号: 3404
• 分子式: C4H11NO3
• 平均质量: 121.13504
• 单一同位素质量: 121.07389322
• SMILES: NC(CO)(CO)CO
• Canonical SMILES: OCC(CO)(CO)N
• InChI: InChI=1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChIKey: LENZDBCJOHFCAS-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 2-amino-2-(hydroxymethyl)propane-1,3-diol
• IUPAC传统名: tris buffer; tris-base
• 别名: 三(羟基甲基)氨基甲烷; 2-氨基-2-(羟甲基)-1,3-丙二醇; Tris 碱; 氨基丁三醇; Trizma® 碱; 缓血酸胺; 三(羟甲基)氨基甲烷, ACS; 三(羟甲基)氨基甲烷; 氨丁三醇; 三羟甲基氨基甲烷; Trizma® 碱; Tris(Hydroxymethyl)Aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; Tris-[hydroxymethyl]amino-methane; TRIS ULTRA PURE GRADE; Tris(hydroxymethyl)aminomethane; ATX Tris buffer; Trizma® base; Sigma 7-9®; Tromethamine; Trometamol; Trometamol; Tromethamine; Tris-[hydroxymethyl]aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; TRIS TECHNICAL GRADE; TRIS, ULTRA PURE; 2-amino-2-(hydroxymethyl)propane-1,3-diol; Tris(hydroxymethyl)aminomethane, ACS; 2-Amino-2-hydroxymethyl-1,3-propanediol; Trometamol, Tromethamine, Tris-[hydroxymethyl]amino-methane; TRIS; TRIS USP GRADE; TRIS; Tris base; Tris buffer; Trizma^TM; Trisamine; THAM; Tromethane; Trometamolum; Tris(hydroxymethyl)aminomethane; Trizma® base

登记号

• CAS号: 77-86-1
• EC号: 201-064-4
• MDL号: MFCD00004679
• Beilstein号: 3535773; 741883
• 默克索引号: 149772
• PubChem SID: 160966844; 24845756; 24900345; 24900405; 46506027; 24899961; 24889887; 24899973; 24855244; 24900399; 24889850; 24852982; 24900061; 24889825; 24900556; 24889854
• PubChem CID: 6503; 3777159
• CHEBI ID: 9754
• ATC码: B05BB03; B05XX02
• CHEMBL: 1200391
• Chemspider ID: 6257
• KEGG ID: D00396
• 美国药典/FDA物质标识码: 023C2WHX2V
• 维基百科标题: Tris

理论计算性质

JChem

• Acid pKa: 14.164156
• 质子受体: 4
• 质子供体: 4
• LogD (pH = 5.5): -5.6058097
• LogD (pH = 7.4): -4.264039
• Log P: -2.712229
• 摩尔折射率: 28.3555 cm^3
• 极化性: 11.566923 Å^3
• 极化表面积: 86.71 Å^2
• 可自由旋转的化学键: 3
• 里宾斯基五规则: true

ALOGPS 2.1

• Log P: -2.54
• LOG S: 0.37
• 溶解度: 3.70e+02 g/l

分子性质

理化性质

• 溶解度: : soluble40 % (w/w), clear, colorless; ~50 g/100 mL (25 °C) in water; H2O: soluble1 M at 20 °C, clear; H2O: soluble1 M, clear, colorless; H2O: soluble4 M at 20 °C, clear, colorless
• 外观: Crystalline; white crystalline; White crystalline powder; white to off-white crystalline; white to very faintly yellow crystalline; yellow tan crystalline
• 熔点: >175-176 °C (448-449 K); 167-172 °C(lit.); 168-171 °C; 168-172 °C; 168-172°C; 171-172 °C; 171-172°C
• 沸点: 219°C; 219-220 °C at 10 mmHg; 219-220 °C/10 mmHg(lit.); 219-220°C/10mm
• 闪点: Non-flammable
• 密度: 1.328g/cm^3; 1.353
• 蒸汽压: Low at ambient temperatures.
• 紫外吸收波长: λ: 230 nm Amax: ≤0.6; λ: 260 nm Amax: ≤0.05; λ: 260 nm Amax: ≤0.10; λ: 260 nm Amax: 0.10; λ: 280 nm Amax: ≤0.04; λ: 280 nm Amax: ≤0.08; λ: 280 nm Amax: 0.08; λ: 430 nm Amax: ≤0.01
• pKa: 8.07; 8.1 (25°C)
• pH值: 10.3-10.8 (0.1 M); 10.5-11.5 (25 °C, 10% in H2O); 10.5-12.0 (1 M in H2O); 10.5-12.0 (25 °C, 4 M in H2O)

安全信息

• 保存条件: Room Temperature (15-30°C); Room Temperature (15-30°C), Desiccate
• 保存注意事项: Hygroscopic; IRRITANT
• RTECS编号: TY2900000
• 欧盟危险性物质标志: 刺激性(Irritant) (Xi)
• 德国WGK号: 2
• 危险公开号: 36/37/38; R:36/37/38; R36 R37 R38
• 安全公开号: 26-36; 26-37; S:20-25-26-37/39; S26 S36
• TSCA收录: false; 是
• GHS危险品标识: GHS07
• GHS警示词: Warning
• 主要危害: Irritant
• NFPA704:
  

  2
• GHS危险声明: H315-H319; H315-H319-H335
• GHS警示性声明: P261-P305 + P351 + P338; P261-P305+P351+P338-P302+P352-P321-P405-P501A; P305 + P351 + P338
• 个人保护装置: dust mask type N95 (US), Eyeshields, Gloves; Eyeshields, Gloves

药理学性质

• 作用靶点: Others

产品相关信息

• 纯度: ≥99%; ≥99% (titration); ≥99.0%; ≥99.0% (T); ≥99.5%; ≥99.7% (T); ≥99.8%; ≥99.8% (T); ≥99.9%; ≥99.9% (titration); 96%; 98%; 99%; 99.0-99.5%; 99.8-100.1%; 99.8-100.1% (Assay, dried basis); 99.9%; 99.95%
• 级别: ACS reagent; Biotechnology Performance Certified; for luminescence; for molecular biology; JIS special grade; Ph Eur; puriss. p.a.; TECHNICAL; ULTRA PURE; ultrapure grade; USP
• 成盐信息: Free Base
• 适用性: cell culture tested; in accordance for luminescence; meets EP, USP testing specifications; meets USP testing specifications
• 燃烧残渣: ≤0.01% (as SO4); ≤0.01% (as SO4) (900°C); ≤0.05% (as SO4)
• 简要说明: aminopeptidase substrate
• 杂质: ≤0.0005% heavy metals (as Pb); ≤0.005% Insoluble matter; ≤0.005% insolubles; ≤0.2% water (Karl Fischer); ≤0.5% water (Karl Fischer); ≤2% water; DNase, RNase, protease, none detected; DNases, none detected; Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; RNases, none detected
• 痕量阳离子: Al: ≤0.0005%; Al: ≤5 mg/kg; As: ≤0.0001%; As: ≤0.1 mg/kg; Ba: ≤0.0005%; Ba: ≤5 mg/kg; Bi: ≤0.0005%; Bi: ≤5 mg/kg; Ca: ≤0.001%; Ca: ≤10 mg/kg; Cd: ≤0.0005%; Cd: ≤5 mg/kg; Co: ≤0.0005%; Co: ≤5 mg/kg; Cr: ≤0.0005%; Cr: ≤5 mg/kg; Cu: ≤0.0005%; Cu: ≤5 mg/kg; Fe: ≤0.0005%; Fe: ≤5 mg/kg; Fe: ≤5 ppm; heavy metals (as Pb): ≤2 ppm; heavy metals (as Pb): ≤5 ppm; K: ≤0.005%; K: ≤50 mg/kg; Li: ≤0.0005%; Li: ≤5 mg/kg; Mg: ≤0.0005%; Mg: ≤5 mg/kg; Mn: ≤0.0005%; Mn: ≤5 mg/kg; Mo: ≤0.0005%; Mo: ≤5 mg/kg; Na: ≤0.005%; Na: ≤50 mg/kg; Ni: ≤0.0005%; Ni: ≤5 mg/kg; Pb: ≤0.0005%; Pb: ≤5 mg/kg; Sr: ≤0.0005%; Sr: ≤5 mg/kg; Zn: ≤0.0005%; Zn: ≤0.5 mg/kg; Zn: ≤5 mg/kg
• 痕量阴离子: chloride (Cl-): ≤0.005%; chloride (Cl-): ≤20 mg/kg; chloride (Cl-): ≤5 mg/kg; sulfate (SO42-): ≤0.005%; sulfate (SO42-): ≤5 mg/kg
• 干燥失重: ≤0.5% loss on drying, 110 °C; ≤0.5% loss on drying, 110°C; ≤1% loss on drying, 110 °C; ≤2% loss on drying, 110 °C
• pH范围: 7 - 9; 7.0 - 9.2
• 产品质量级别: ELITE
• 品质说明: certified by BAM, according to ISO 17025
• λ: 10 % in H2O; 4 M in H2O
• 适用pH范围: 7 - 9
• 药典: testing & handling conforms to Pharmacopeia
• 产品线: BioUltra; BioXtra
• abs.: / passes test; A1M/260, H2O ≤0.025; A1M/280, H2O ≤0.020; A40%/290 ≤0.05; A40%/290 ≤0.4
• 线性分子式: NH2C(CH2OH)3
• Empirical Formula (Hill Notation): C4H11NO3

详细说明

MP Biomedicals - 02103133

Ultra Pure Grade
Purity: 99.95% min.
Buffering pH range 7.0-9.0
pKa at 25°C = 8.1
Excellent biochemical and biological buffer for all applications where ultra high purity is required. MP offers a complete selection of Biological Buffers including: BES, BICINE, BIS-TRIS, HEPES, MOPS, PIPES, And many more...

MP Biomedicals - 02195605

USP Grade
Purity: 99.95% min.
Buffering pH range 7.0-9.0
pKa at 25°C = 8.1
Excellent biochemical and biological buffer for all applications where ultra high purity is required.

MP Biomedicals - 02103132

Technical Grade
Purity: 96% min.
Buffering pH range 7.0-9.0
pKa at 25°C = 8.1
This grade of tris is suitable for use as a standard biological buffer in less critical applications where economy is an important consideration.

MP Biomedicals - 04819620

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Purity: 99.9% Heavy metals (as Pb): <5 ppm O.D. (260nm) of a 5% saturated solution: <0.1 pKa at 20°C = 8.1 Biological buffer reagent.

MP Biomedicals - 02152176

Purity: 99.0-99.5%
Buffering pH range 7.0-9.0 pKa at 25°C = 8.1
This grade of tris is excellent where purity and value are both important. It is superior to our technical grade tris and less expensive than out ultra pure material. This grade will suffice for most buffer applications.

MP Biomedicals - 04819638

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Purity: 99.9% Heavy metals (as Pb): <5 ppm O.D. (260nm) of a 5% saturated solution: <0.1 pKa at 20°C = 8.1 Biological buffer reagent.

MP Biomedicals - 04819623

(Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane) Purity: 99.9% Heavy metals (as Pb): <5 ppm O.D. (260nm) of a 5% saturated solution: <0.1 pKa at 20°C = 8.1 Biological buffer reagent.

MP Biomedicals - 02194557

(Trometamol, Tromethamine, Tris-[hydroxymethyl]amino- methane) Cell Culture Reagent Purity: 99.95% min. Buffering pH range 7.0-9.0 pKa at 25°C = 8.1 Excellent biochemical and biological buffer for all applications where ultra high purity is required.

MP Biomedicals - 02194855

(Trometamol, Tromethamine, Tris-[hydroxymethyl]amino- methane) Molecular Biology Reagent Purity: 99.95% min. Buffering pH range 7.0-9.0 pKa at 25°C = 8.1 Excellent biochemical and biological buffer for all molecular biology applications.

Sigma Aldrich - 93350

Other Notes
便于将 Trizma 产品规格与 Trizma 规格表进行比较。
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T1410

Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 93286

Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T4661

Analysis Note
未经过热原测试。可提供包含批次特定信息的分析证书。
Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
包装
所有规格与 T1503 相同。在生物技术方面，用于诊断或制造。请咨询最小起订量。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T6066

Application
未经过欧洲药典专论中所述肠外应用的测试。
Other Notes
便于将 Trizma 产品规格与 Trizma 规格表进行比较。
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T6687

Other Notes
便于将 Trizma 产品规格与 Trizma 规格表进行比较。

Sigma Aldrich - 93362

Other Notes
Running buffer component in non-denaturing agarose gel electrophoresis1; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis2; DNA digest analysis with capillary electrophoresis3.
Easily compare specifications for Trizma products with the Trizma specification table.
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 93304

Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 30-5025

Suitability
for biological purposes

Sigma Aldrich - T1378

General description
40% (w/w) 的水溶液为透明的无色至微黄色液体。
Other Notes
便于将 Trizma 产品的规格与 Trizma 规格表进行比较。
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Sigma 7-9 注册商标 Sigma-Aldrich Co. LLC
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 08656

Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 33742

Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 252859

Other Notes
便于将 Trizma 产品规格与 Trizma 规格表进行比较。
包装
100, 500 g in poly bottle

Sigma Aldrich - T87602

包装
1 kg in poly bottle
250 g in poly bottle
3 kg in poly drum

Sigma Aldrich - 93349

Other Notes
在高摩尔浓度时可用于通过凝胶电泳测定多肽和蛋白质的分子量1；蛋白质在醋酸纤维素膜上的电泳2；校正聚丙烯酰胺凝胶柱，用于通过毛细管电泳分离寡核苷酸3；单碳酸钠熔融少量地质样品中微量氟的测定4。
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - T6791

Other Notes
便于将 Trizma 产品规格与 Trizma 规格表进行比较。
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 04577

Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 93440

Analysis Note
精确浓度、有效期、可溯源性以及详细认证信息，请见每个包装内的证书和认证报告。标签上标明含量和有效期。
Other Notes
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 154563

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
缓冲剂和 pH 基准。20°C 时 pKa 为 8.3。1药用辅料。2,3
包装
1 kg in poly bottle
5 g in glass bottle
100, 500 g in poly bottle
5 kg in poly drum

Sigma Aldrich - 30-5020

Suitability
for electrophoresis

Sigma Aldrich - 21685

包装
6 bottles for the preparation of 1 L ready-to-use solution (each)

Sigma Aldrich - T1503

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
General description
40% (w/w) 的水溶液是澄清无色的。
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
所有缓冲液的 pH 值与温度和浓度都有关。对于 Tris 缓冲液，温度每降低 1°C，pH 增加约 0.03 个单位，浓度每稀释 10 倍，pH 降低 0.03-0.05 个单位。 对于精确应用，使用带有玻璃/甘汞复合电极的经仔细校正的 pH 计。
包装
1 kg in poly bottle
25, 100, 250, 500 g in poly bottle
5, 10, 25, 50 kg in poly drum
法律信息
Trizma 注册商标 Sigma-Aldrich Co. LLC
Protocols & Applications
Ensure correct concentrations of buffer solutions with the Buffer Calculator

DrugBank - DB03754

• Drug Groups: experimental
• Description: An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)

参考文献

• Widely used biological buffer.