[2,3-bis(octadec-9-enoyloxy)propyl]trimethylazanium methyl sulfate

• ChemBase编号: 156126
• 分子式: C43H83NO8S
• 平均质量: 774.18602
• 单一同位素质量: 773.58393962
• SMILES: CCCCCCCC/C=C/CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC/C=C/CCCCCCCC.COS(=O)(=O)[O-]
• Canonical SMILES: COS(=O)(=O)[O-].CCCCCCCC/C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC/C=C/CCCCCCCC)C[N+](C)(C)C
• InChI: InChI=1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1
• InChIKey: RSMRWWHFJMENJH-UHFFFAOYSA-M

名称和登记号

名称

• IUPAC标准名: [2,3-bis(octadec-9-enoyloxy)propyl]trimethylazanium methyl sulfate
• IUPAC传统名: [2,3-bis(octadec-9-enoyloxy)propyl]trimethylazanium methyl sulfate(1-)
• 别名: N-(2,3-Dioleoyloxy-1-propyl)trimethylammonium methyl sulfate; Transfection reagent; DOTAP methosulfate

登记号

• CAS号: 144189-73-1
• MDL号: MFCD00274414
• PubChem SID: 24889327; 162250264
• PubChem CID: 22996898

理论计算性质

• 质子受体: 2
• 质子供体: 0
• LogD (pH = 5.5): 9.603935
• LogD (pH = 7.4): 9.603935
• Log P: 9.603935
• 摩尔折射率: 215.9765 cm^3
• 极化性: 80.64132 Å^3
• 极化表面积: 52.6 Å^2
• 可自由旋转的化学键: 38
• 里宾斯基五规则: false

分子性质

安全信息

• 德国WGK号: 3
• 个人保护装置: Eyeshields, Gloves
• 保存温度: 2-8°C

产品相关信息

• 级别: for molecular biology
• Empirical Formula (Hill Notation): C43H83NO8S

详细说明

Sigma Aldrich - 90189

Application
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
liposomal transfection reagent (1mg/ml)
Other Notes
Mediates transfection of mammalian cells1