λ2-ruthenium(2+) ion bis(2-(pyridin-2-yl)pyridine) bis(hexafluoro-λ5-phosphanuide) 2,5-dioxopyrrolidin-1-yl 2-(4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}pyridin-2-yl)pyridine-4-carboxylate

• ChemBase编号: 155893
• 分子式: C40H30F12N8O8P2Ru
• 平均质量: 1141.7133604
• 单一同位素质量: 1142.05137086
• SMILES: c1ccnc(c1)c1ccccn1.c1ccnc(c1)c1ccccn1.c1cnc(cc1C(=O)ON1C(=O)CCC1=O)c1cc(ccn1)C(=O)ON1C(=O)CCC1=O.F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.[Ru+2]
• Canonical SMILES: c1ccc(nc1)c1ccccn1.c1ccc(nc1)c1ccccn1.F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.O=C1CCC(=O)N1OC(=O)c1ccnc(c1)c1nccc(c1)C(=O)ON1C(=O)CCC1=O.[Ru+2]
• InChI: InChI=1S/C20H14N4O8.2C10H8N2.2F6P.Ru/c25-15-1-2-16(26)23(15)31-19(29)11-5-7-21-13(9-11)14-10-12(6-8-22-14)20(30)32-24-17(27)3-4-18(24)28;2*1-3-7-11-9(5-1)10-6-2-4-8-12-10;2*1-7(2,3,4,5)6;/h5-10H,1-4H2;2*1-8H;;;/q;;;2*-1;+2
• InChIKey: HIPJTYPALXAMBX-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: λ^2-ruthenium(2+) ion bis(2-(pyridin-2-yl)pyridine) bis(hexafluoro-λ^5-phosphanuide) 2,5-dioxopyrrolidin-1-yl 2-(4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}pyridin-2-yl)pyridine-4-carboxylate
• IUPAC传统名: λ^2-ruthenium(2+) ion bis(bipyridyl) 2,5-dioxopyrrolidin-1-yl 2-(4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}pyridin-2-yl)pyridine-4-carboxylate dihexafluorophosphate
• 别名: Ru(bpy)2(dcbpy-di O-Su-ester)(PF6)2; Bis(2,2′-bipyridine)-4,4′-dicarboxybipyridine-ruthenium di(N-succinimidyl ester) bis(hexafluorophosphate)

登记号

• CAS号: 160525-49-5
• MDL号: MFCD02683416
• PubChem SID: 162250031; 24890332
• PubChem CID: 16218821

理论计算性质

• Acid pKa: 17.396233
• 质子受体: 8
• 质子供体: 0
• LogD (pH = 5.5): 0.41113597
• LogD (pH = 7.4): 0.4111632
• Log P: 0.41116357
• 摩尔折射率: 101.7564 cm^3
• 极化性: 40.69322 Å^3
• 极化表面积: 153.14 Å^2
• 可自由旋转的化学键: 9
• 里宾斯基五规则: false

分子性质

理化性质

• 荧光: λex 458 nm; λem 647 nm in 0.1 M phosphate pH 7.0

安全信息

• 德国WGK号: 3
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 保存温度: -20°C

产品相关信息

• 适用性: suitable for fluorescence
• 运输包装: dry ice
• 产品线: BioReagent
• Empirical Formula (Hill Notation): C40H30F12N8O8P2Ru

详细说明

Sigma Aldrich - 96632

Application
Ruthenium-ligand belonging to a new class of long-lived fluorophores for protein hydrodynamics.
Features and Benefits
Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds. These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes’ shifts. In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.