2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol

• ChemBase编号: 155574
• 分子式: C14H27N3O11
• 平均质量: 413.37768
• 单一同位素质量: 413.1645587
• SMILES: C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O
• Canonical SMILES: OC(=O)CN(CC(=O)O)CCN(CC(=O)O)CC(=O)O.OCC(CO)(CO)N
• InChI: InChI=1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• InChIKey: VLEIUWBSEKKKFX-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid; 2-amino-2-(hydroxymethyl)propane-1,3-diol
• IUPAC传统名: edta; tris buffer
• 别名: TE 缓冲液 溶液; TRIS--EDTA solution; TE buffer solution; Tris-EDTA buffer solution

登记号

• EC号: 231-791-2
• MDL号: MFCD00236359
• PubChem SID: 162249712; 24888617
• PubChem CID: 16218629

理论计算性质

• Acid pKa: 1.486185
• 质子受体: 10
• 质子供体: 4
• LogD (pH = 5.5): -10.586659
• LogD (pH = 7.4): -14.721412
• Log P: -5.221795
• 摩尔折射率: 62.3456 cm^3
• 极化性: 24.69443 Å^3
• 极化表面积: 155.68 Å^2
• 可自由旋转的化学键: 14
• 里宾斯基五规则: true

分子性质

理化性质

• 密度: 1.050-1.070 g/mL at 20 °C
• 折射率: n20/D 1.352-1.354; n20/D 1.360
• 紫外吸收波长: λ: 260 nm Amax: 0.03; λ: 260 nm Amax: 0.04; λ: 280 nm Amax: 0.01; λ: 280 nm Amax: 0.05
• pH值: 7.4±0.2 (25 °C); 8.0±0.1 (25 °C); 8.8-9.2 (25 °C)

安全信息

• 欧盟危险性物质标志: 刺激性(Irritant) (Xi)
• 德国WGK号: 1; 2; nwg
• 危险公开号: 41
• 安全公开号: 26-39
• GHS危险品标识: GHS05
• GHS警示词: Danger
• GHS危险声明: H318
• GHS警示性声明: P280-P305 + P351 + P338
• 个人保护装置: Eyeshields, Gloves

产品相关信息

• 浓度: 0.23-0.27 M; 100 ×
• 级别: for molecular biology
• 杂质: DNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; RNases, none detected
• 痕量阳离子: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• Foreign Activity: DNase, RNase, none detected
• 无菌消毒: 0.2 μm filtered
• 品质说明: 100× concentrate
• λ: neat
• 产品线: BioUltra
• abs.: absorption/260 nm cut-off

详细说明

Sigma Aldrich - T9285

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Other Notes
1.0M Tris-HCl，pH 约8.0，含 0.1M EDTA

Sigma Aldrich - 86377

Physical form
1.0M TRIS HCl，pH 8.0±0.1 (25°C)；0.1M EDTA.Na2

Sigma Aldrich - 98306

Physical form
0.25 M EDTA sodium salt; 0.25 M TRIS hydrochloride in water

Sigma Aldrich - 93302

Physical form
10mM Tris-HCl；1mM EDTA

Sigma Aldrich - 93283

Other Notes
该缓冲剂为 10mM Tris-HCl, 1mM EDTA 二钠，pH 8.0。