acetic acid tert-butyl N-[(1S)-1-{[(1S)-1-{[(1S)-4-carbamimidamido-1-[(5-methyl-2-oxo-2H-chromen-7-yl)carbamoyl]butyl]carbamoyl}-2-hydroxyethyl]carbamoyl}-2-phenylethyl]carbamate

• ChemBase编号: 132837
• 分子式: C35H47N7O10
• 平均质量: 725.78858
• 单一同位素质量: 725.33844074
• SMILES: Cc1cc(cc2c1ccc(=O)o2)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1ccccc1)NC(=O)OC(C)(C)C.CC(=O)O
• Canonical SMILES: CC(=O)O.OC[C@@H](C(=O)N[C@H](C(=O)Nc1cc(C)c2c(c1)oc(=O)cc2)CCCNC(=N)N)NC(=O)[C@H](Cc1ccccc1)NC(=O)OC(C)(C)C
• InChI: InChI=1S/C33H43N7O8.C2H4O2/c1-19-15-21(17-26-22(19)12-13-27(42)47-26)37-28(43)23(11-8-14-36-31(34)35)38-30(45)25(18-41)39-29(44)24(16-20-9-6-5-7-10-20)40-32(46)48-33(2,3)4;1-2(3)4/h5-7,9-10,12-13,15,17,23-25,41H,8,11,14,16,18H2,1-4H3,(H,37,43)(H,38,45)(H,39,44)(H,40,46)(H4,34,35,36);1H3,(H,3,4)/t23-,24-,25-;/m0./s1
• InChIKey: WGWZNYKOUXOZTC-NAGNLMCHSA-N

名称和登记号

名称

• IUPAC标准名: acetic acid tert-butyl N-[(1S)-1-{[(1S)-1-{[(1S)-4-carbamimidamido-1-[(5-methyl-2-oxo-2H-chromen-7-yl)carbamoyl]butyl]carbamoyl}-2-hydroxyethyl]carbamoyl}-2-phenylethyl]carbamate
• IUPAC传统名: acetic acid tert-butyl N-[(1S)-1-{[(1S)-1-{[(1S)-4-carbamimidamido-1-[(5-methyl-2-oxochromen-7-yl)carbamoyl]butyl]carbamoyl}-2-hydroxyethyl]carbamoyl}-2-phenylethyl]carbamate
• 别名: Tryptase 来源于人类肺; Tryptase from human lung

登记号

• MDL号: MFCD00272854
• PubChem SID: 162227114
• PubChem CID: 5311489
• Enzyme Commission Number: 3.4.21.59 ( BRENDA; IUBMB )

理论计算性质

• Acid pKa: 11.442299
• 质子受体: 9
• 质子供体: 8
• LogD (pH = 5.5): -1.1180084
• LogD (pH = 7.4): -1.1110796
• Log P: 0.56147397
• 摩尔折射率: 188.3627 cm^3
• 极化性: 67.606964 Å^3
• 极化表面积: 234.06 Å^2
• 可自由旋转的化学键: 16
• 里宾斯基五规则: false

分子性质

理化性质

• 外观: buffered aqueous solution

安全信息

• 德国WGK号: 1
• 保存温度: -20°C

药理学性质

• 相关基因信息: human ... TPSAB1(7177), TPSB2(64499), TPSD1(23430), TPSG1(25823)

产品相关信息

• 运输包装: dry ice

详细说明

Sigma Aldrich - T7063

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Quality
Highly purified
Unit Definition
One unit will hydrolyze 1.0 μmole of N-benzoyl DL-arginine p-nitroanilide per minute at pH 7.8 at 37 °C.
Physical form
Solution in 1 M NaCl, 50 mM sodium acetate, pH 5.0, containing 0.01% sodium azide
Biochem/physiol Actions
Tryptase is a member of the serine protease S1 family. It is the predominant neutral protease of the mast cell granules. Within the mast cell granule it exists as a heparin-stabilized active tetramer. Stabilization is a result of the high negative charge density of the glycosaminoglycan. This stabilization activity is observed with heparins with a MW greater than 6 kDa as well as other glycosaminoglycans such as dextran sulfate or chondroitin sulfates. Removal of heparin results in dissociation of the tetramer and inactivation of the enzyme. High concentrations of NaCl will result in the dissociation of heparin. Tryptase is released from the mast cell as a result of the degranulation response during anaphylaxis. In addition, several tryptase genes and alleles (α, β, γ & δ) have been identified in various tissues and circulating in blood. Pro-β-tryptase is thought to be the constituative circulating form in blood. The biological function of tryptase is unknown. However it has been reported to catalyze the activation of complement C3, convert prostromelysin to stromelysin (MMP-3), and cleave fibrinogen resulting in a loss of clottting potential. Tryptase also degrades fibronectin, calcitonin gene-related peptide, vasoactive intestinal peptide,and kininogen.
General description
Tryptase is a glycoprotein released from mast cells during anaphylaxis, which performs a number of functions including catalyzing the activation of complement C3, converting prostromelysin to stromelysin (MMP-3), and cleaving fibrinogen resulting in a loss of clotting potential.
Physical properties
Molecular Weight: ~135 kDa (Human)(Non-covalently linked tetramer with two sets of dissimilar subunits possibly resulting from heterogeneity in N-linked glycosylation and existence of a & b isoforms sequences in human lung). 31-33 kDa (Monomer MW)