{[5-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}({[5-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]oxy})phosphinic acid amine

• ChemBase编号: 132160
• 分子式: C18H26N5O14P
• 平均质量: 567.397901
• 单一同位素质量: 567.12138717
• SMILES: c1cn(c(=O)[nH]c1=O)C1C(C(C(O1)COP(=O)(O)OC1C(OC(C1O)n1ccc(=O)[nH]c1=O)CO)O)O.N
• Canonical SMILES: OCC1OC(C(C1OP(=O)(OCC1OC(C(C1O)O)n1ccc(=O)[nH]c1=O)O)O)n1ccc(=O)[nH]c1=O.N
• InChI: InChI=1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• InChIKey: VPXDHZMLJOJKOX-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: {[5-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}({[5-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]oxy})phosphinic acid amine
• IUPAC传统名: [5-(2,4-dioxo-3H-pyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy[5-(2,4-dioxo-3H-pyrimidin-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]oxyphosphinic acid amine
• 别名: Urate oxidase; Urate:O2 oxidoreductase; Uricase from Arthrobacter globiformis; Uricase from Bacillus fastidiosus; UpU; Uridylyl(3′→5′)uridine ammonium salt; Urate: oxygen oxoreductase; Uricase from Candida sp.

登记号

• CAS号: 27552-95-0; 9002-12-4
• EC号: 232-655-5
• MDL号: MFCD00082126; MFCD00058217
• PubChem SID: 24900619; 24889980; 24900663; 162226437; 24900666
• PubChem CID: 14228836
• Enzyme Commission Number: 1.7.3.3 ( BRENDA; IUBMB )

理论计算性质

• Acid pKa: 1.8328414
• 质子受体: 12
• 质子供体: 7
• LogD (pH = 5.5): -6.412375
• LogD (pH = 7.4): -6.437917
• Log P: -4.0575128
• 摩尔折射率: 112.2251 cm^3
• 极化性: 45.211254 Å^3
• 极化表面积: 253.96 Å^2
• 可自由旋转的化学键: 8
• 里宾斯基五规则: false

分子性质

理化性质

• 外观: lyophilized powder

安全信息

• RTECS编号: YU7100000
• 德国WGK号: -; 3
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 保存温度: -20°C

产品相关信息

• 产品质量级别: PREMIUM
• 基因表达载体: expressed in E. coli
• 品质说明: lyophilized
• Mol. Weight: mol wt 35 kDa by SDS-PAGE

详细说明

Sigma Aldrich - U0880

Biochem/physiol Actions
Suitable for the determination of uric acid in clinical analysis.
Unit Definition
One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.
Physical form
Powder contains citrate.

Sigma Aldrich - 94310

Other Notes
For the determination of uric acid in biological fluids1; In the spectrophotometric determination of purines and purine nucleosides2.
Unit Definition
1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Sigma Aldrich - U7128

Unit Definition
One unit will convert 1.0 μmole of uric acid to allantoin per min at pH 9.0 at 25 °C.
Biochem/physiol Actions
Molecular weight: 4 × ~32 KDa (tetramer)Isoelectric point: is 4.64KM value (uric acid as substrate): ~ 0.01 mM The optimum pH for activity: 8.5-9.5The optimum pH for stability: 6.0-9.5