2',7'-dichloro-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthene]-3-one

• ChemBase编号: 109177
• 分子式: C20H10Cl2O5
• 平均质量: 401.1964
• 单一同位素质量: 399.99052878
• SMILES: Oc1cc2c(cc1Cl)C1(OC(=O)c3ccccc13)c1cc(Cl)c(O)cc1O2
• Canonical SMILES: O=C1OC2(c3c1cccc3)c1cc(Cl)c(cc1Oc1c2cc(Cl)c(c1)O)O
• InChI: InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
• InChIKey: VFNKZQNIXUFLBC-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 2',7'-dichloro-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthene]-3-one
• IUPAC传统名: dichlorofluorescein
• 别名: 2′,7′-二氯荧光素; 2,7-二氯荧光素; 2′,7′-二氯荧光素 溶液; DICHLOROFLUORESCEIN; 2′,7′-Dichlorofluorescein; 2',7'-Dichlorofluorescein; 2′,7′-Dichlorofluorescein solution

登记号

• CAS号: 76-54-0
• EC号: 200-968-6
• MDL号: MFCD00005047
• Beilstein号: 58009
• PubChem SID: 24894041; 162096140; 24845334; 24865700; 24861978
• PubChem CID: 64944
• CHEBI ID: 51596
• CHEMBL: 1908059
• Chemspider ID: 58471
• 维基百科标题: Dichlorofluorescein

理论计算性质

• Acid pKa: 7.135351
• 质子受体: 3
• 质子供体: 2
• LogD (pH = 5.5): 5.078509
• LogD (pH = 7.4): 4.528039
• Log P: 5.0884323
• 摩尔折射率: 100.8297 cm^3
• 极化性: 38.246376 Å^3
• 极化表面积: 75.99 Å^2
• 可自由旋转的化学键: 0
• 里宾斯基五规则: false

分子性质

理化性质

• 溶解度: alcohol: passes test
• 外观: crystalline; orange to red-brown, powder
• 熔点: >300°C dec.; 280 °C (dec.)(lit.)
• 闪点: 12 °C; 53.6 °F
• 密度: 0.79 g/mL at 20 °C
• 紫外吸收波长: 509 nm; λmax 509 nm
• 荧光: λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0

安全信息

• 欧盟危险性物质标志: 易燃性(Flammable) (F); 刺激性(Irritant) (Xi)
• 联合国危险货物编号: 1219
• 德国WGK号: 2; 3
• 联合国危险货物等级: 3
• 联合国危险货物包装类别(PG): 2
• 危险公开号: 11-36-67; 36; 36/37/38
• 安全公开号: 16-26; 26; 26-36
• TSCA收录: 是
• GHS危险品标识: GHS02; GHS07
• GHS警示词: Danger; Warning
• GHS危险声明: H225-H319-H336; H315-H319-H335; H319
• GHS警示性声明: P210-P261-P305 + P351 + P338; P261-P305 + P351 + P338; P280-P264-P305+P351+P338-P337+P313
• 个人保护装置: dust mask type N95 (US), Eyeshields, Gloves; Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
• RID/ADR: UN 1219 3/PG 2

产品相关信息

• 纯度: ~90% (TLC); ≥90% (T)
• 级别: ACS reagent; indicator
• 适用性: passes test for adsorption indicator; suitable for fluorescence
• 产品线: BioReagent
• Empirical Formula (Hill Notation): C20H10Cl2O5

详细说明

MP Biomedicals - 05210122

MP Biomedicals Rare Chemical collection

Sigma Aldrich - 35848

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Application example:Dip reagent for the fluorimetric determination of vicinal diols, glycosides and phenols.Solution A: Saturated solution of lead tetraacetate (~2%) (Fluka-No. 15370) in glacial acetic acidSolution B: 2',7'-Dichlorofluorescein in ethanol (0.2-1%)Mix 5 mL solution A with 5 mL solution B and bring to 200 mL with toluene, just prior to using. Solution should be freshly prepared each time. Dipping time: 10 sec.
Other usage examples: determination of carbohydrates by fluorescence densitometry after TLC; determination of H2O2 by chemiluminescence; fluorescent additive used in indirect fluorimetric detection in OTC-HPLC

Sigma Aldrich - D6665

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.

Sigma Aldrich - 02591

Application
即用型色谱喷雾和浸入显色剂

Sigma Aldrich - 410217

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
包装
1, 10 g in glass bottle