3,8-diamino-5-{3-[diethyl(methyl)azaniumyl]propyl}-6-phenylphenanthridin-5-ium diiodide

• ChemBase编号: 105971
• 分子式: C27H34I2N4
• 平均质量: 668.3946
• 单一同位素质量: 668.08729311
• SMILES: CC[N+](C)(CC)CCC[n+]1c2cc(ccc2c2ccc(cc2c1c1ccccc1)N)N.[I-].[I-]
• Canonical SMILES: CC[N+](CCC[n+]1c(c2ccccc2)c2cc(N)ccc2c2c1cc(N)cc2)(CC)C.[I-].[I-]
• InChI: InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• InChIKey: XJMOSONTPMZWPB-UHFFFAOYSA-M

名称和登记号

名称

• IUPAC标准名: 3,8-diamino-5-{3-[diethyl(methyl)azaniumyl]propyl}-6-phenylphenanthridin-5-ium diiodide
• IUPAC传统名: propidium diiodide
• 别名: 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide; PROPIDIUM IODIDE; 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide; Propidium iodide; 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide; 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide; Propidium iodide solution; 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium Iodide; [3-(3,8-Diamino-6-phenyl-5-phenanthridinio)propyl]diethylmethylammonium Diiodide; 3,8-Diamino-5-(diethylmethylaminopropyl)-6-phenylphenanthridinium Diiodide; Propidium Diiodide

登记号

• CAS号: 25535-16-4
• EC号: 231-791-2; 247-081-0
• MDL号: MFCD00011921
• Beilstein号: 3843838
• PubChem SID: 24898563; 24898510; 162093131
• PubChem CID: 104981
• CHEBI ID: 51240
• CHEMBL: 345124
• Chemspider ID: 94732
• 维基百科标题: Propidium_iodide

理论计算性质

• 质子受体: 2
• 质子供体: 2
• LogD (pH = 5.5): -4.6443014
• LogD (pH = 7.4): -4.6402345
• Log P: -4.640182
• 摩尔折射率: 145.5442 cm^3
• 极化性: 54.351612 Å^3
• 极化表面积: 55.92 Å^2
• 可自由旋转的化学键: 7
• 里宾斯基五规则: false

分子性质

理化性质

• 溶解度: DMSO; Methanol
• 外观: Dark Red Solid; solution (1.0 mg/ml in water)
• 熔点: 220-225 °C (dec.)(lit.); 220-225°C; 224-228°C
• 紫外吸收波长: λmax 493 nm
• 荧光: λex 482 nm; λem 608 nm (pH 7.2); λex 530 nm; λem 620 nm in 0.1 M phosphate pH 7.0 (bound to DNA); λex 540 nm; λem 608 nm (bound to DNA)

安全信息

• 保存条件: 2-8°C, Desiccate, Protect from light; Refrigerator
• RTECS编号: SF7949600
• 欧盟危险性物质标志: 刺激性(Irritant) (Xi); 有害性(Harmful) (Xn)
• 德国WGK号: 3
• 危险公开号: 36/37/38; R:20/21/22
• 安全公开号: 26; S:26-36-46
• GHS危险品标识: GHS07
• GHS警示词: Warning
• GHS危险声明: H315-H319-H335
• GHS警示性声明: P261-P305 + P351 + P338
• 个人保护装置: dust mask type N95 (US), Eyeshields, Gloves; Eyeshields, Gloves
• 保存温度: 2-8°C

产品相关信息

• 纯度: ≥90.0% (HPLC); ≥94% (HPLC); ≥94.0% (HPLC); ≥98%; 95%
• 浓度: 1 mg/mL in H2O
• 级别: for fluorescence
• Empirical Formula (Hill Notation): C27H34I2N4

详细说明

MP Biomedicals - 02195458

Red powder
Reagent for the fluorescent staining of nucleic acids.
POSSIBLE CARCINOGEN!

Sigma Aldrich - P4170

Application
Fluorescent stain for nucleic acids. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

Sigma Aldrich - 70335

Application
Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

Sigma Aldrich - P4864

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

Toronto Research Chemicals - P770500

Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells

参考文献

• Karlsson, R., et al.: Anal. Biochem., 278, 1 (2000)
• Lundqvist, T., et al.: Curr. Opin Drug Discov. Dev., 8, 513 (2000)
• Elinder, M., et al.: J. Biomol. Screen, 14, 395 (2000)
• Boettcher, A., et al.: J. Biomol. Screen, 2010, 15, 1029 (2000)