4'-phenyl-3H,3'H-spiro[2-benzofuran-1,2'-furan]-3,3'-dione

• ChemBase编号: 105940
• 分子式: C17H10O4
• 平均质量: 278.2589
• 单一同位素质量: 278.0579088
• SMILES: O=C1OC2(OC=C(C2=O)c2ccccc2)c2ccccc12
• Canonical SMILES: O=C1OC2(c3c1cccc3)OC=C(C2=O)c1ccccc1
• InChI: InChI=1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
• InChIKey: ZFKJVJIDPQDDFY-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 4'-phenyl-3H,3'H-spiro[2-benzofuran-1,2'-furan]-3,3'-dione
• IUPAC传统名: fluorescamine
• 别名: 荧光胺; 4-Phenylspiro[furan-2(3H)-1'-(3'-H)-Isobenzofuran]-3,3'-dione; FLUORESCAMINE; 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione; Fluorescamine; Fluram

登记号

• CAS号: 38183-12-9
• EC号: 253-814-5
• MDL号: MFCD00005928
• Beilstein号: 921143
• 默克索引号: 144158
• PubChem SID: 24894984; 162092857
• PubChem CID: 37927
• 维基百科标题: Fluorescamine

理论计算性质

• 质子受体: 3
• 质子供体: 0
• LogD (pH = 5.5): 3.975393
• LogD (pH = 7.4): 3.975393
• Log P: 3.975393
• 摩尔折射率: 75.1287 cm^3
• 极化性: 28.987541 Å^3
• 极化表面积: 52.6 Å^2
• 可自由旋转的化学键: 1
• 里宾斯基五规则: true

分子性质

理化性质

• 溶解度: acetone: soluble50 mg/mL
• 外观: off-white to yellow powder; Powder
• 熔点: 148-158°C; 153-157 °C(lit.); 153–157 °C; 155-158°C
• 紫外吸收波长: λmax 306 nm

安全信息

• 保存条件: Room Temperature (15-30°C)
• 德国WGK号: 3
• TSCA收录: 否
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

产品相关信息

• 纯度: ≥98% (TLC); ≥98.0%; 98%
• 产品质量级别: PREMIUM
• Empirical Formula (Hill Notation): C17H10O4

详细说明

MP Biomedicals - 02195183

Off-white to yellow powder.
Reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines.^1,2. Also used for fluorometric assays of some proteolytic enzymes.^3

Sigma Aldrich - F9015

Application
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses.
包装
1 g in glass bottle
100, 250 mg in glass bottle

Sigma Aldrich - 201650

Application
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses.
包装
100 mg in glass bottle

Sigma Aldrich - 11-0398

Application
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses.
Suitability
for HPLC

参考文献

• Weigele, M., et al., J. Am. Chem. Soc. , 94 : 5927 (1972).
• Udenfriend, S., et al., Science , 178 : 871 (1972).
• Sogowa, K. and Takahashi, K.J., Biochem. (Tokyo) , 83 : 1783 (1978).