N-(2-{[2-(prop-2-enamido)ethyl]disulfanyl}ethyl)prop-2-enamide

• ChemBase编号: 105512
• 分子式: C10H16N2O2S2
• 平均质量: 260.37624
• 单一同位素质量: 260.06531976
• SMILES: C=CC(=O)NCCSSCCNC(=O)C=C
• Canonical SMILES: C=CC(=O)NCCSSCCNC(=O)C=C
• InChI: InChI=1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)
• InChIKey: DJVKJGIZQFBFGS-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: N-(2-{[2-(prop-2-enamido)ethyl]disulfanyl}ethyl)prop-2-enamide
• IUPAC传统名: N-(2-{[2-(prop-2-enamido)ethyl]disulfanyl}ethyl)prop-2-enamide
• 别名: N,N'-双(丙稀酰)胱胺; 2,2′-(Bisacrylamino)diethyl disulfide; BAC; Bis(2-acrylamidoethyl) disulfide; N,N′-Bis(acryloyl)cystamine; N,N'-Bis(acryloyl)cystamine; N,N'-bis-Acrylylcystamine; N,N'-bis(Acryloyl)cystamine; bis-Acrylylcystamine: BAC; N,N'-CYSTAMINE-bis-ACRYLAMIDE

登记号

• CAS号: 60984-57-8
• EC号: 262-546-8
• MDL号: MFCD00036225
• PubChem SID: 162092355; 24890926
• PubChem CID: 100602

理论计算性质

• Acid pKa: 15.30914
• 质子受体: 2
• 质子供体: 2
• LogD (pH = 5.5): 0.39751455
• LogD (pH = 7.4): 0.39751583
• Log P: 0.39751586
• 摩尔折射率: 71.395 cm^3
• 极化性: 27.262354 Å^3
• 极化表面积: 58.2 Å^2
• 可自由旋转的化学键: 9
• 里宾斯基五规则: true

分子性质

理化性质

• 外观: Powder, Packaged under argon
• 熔点: 121-125 °C; 123-124°C

安全信息

• 保存条件: 0°C, Desiccate
• 欧盟危险性物质标志: 刺激性(Irritant) (Xi)
• 德国WGK号: 3
• 危险公开号: 36/37/38
• 安全公开号: 26-36; 26-37
• TSCA收录: 否
• GHS危险品标识: GHS07
• GHS警示词: Warning
• GHS危险声明: H315-H319-H335
• GHS警示性声明: P261-P305 + P351 + P338; P261-P305+P351+P338-P302+P352-P321-P405-P501A
• 个人保护装置: dust mask type N95 (US), Eyeshields, Gloves
• 保存温度: -20°C; 2-8°C

产品相关信息

• 纯度: ≥97.0% (HPLC); 98%
• 适用性: in accordance for electrophoresis test; suitable for electrophoresis
• 痕量阳离子: Ca: ≤1000 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Mg: ≤50 mg/kg; Mn: ≤5 mg/kg; Na: ≤2000 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• 产品线: BioReagent; BioXtra
• Empirical Formula (Hill Notation): C10H16N2O2S2

详细说明

MP Biomedicals - 02190555

Disulfide containing cross-linker. After electrophoresis, BAC cross-linked gel may be dissolved by addition of a reducing agent such as dithiothreitol or 2-mercaptoethanol. Excellent cross-linker for recovery of material from the gel under physiological conditions.

Sigma Aldrich - A4929

Application
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Sigma Aldrich - 14460

Application
BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

参考文献

• Anal. Biochem. , 76 : 37-44 (1976).