2-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid

• ChemBase编号: 102806
• 分子式: C6H13NO5
• 平均质量: 179.17112
• 单一同位素质量: 179.07937252
• SMILES: OCC(CO)(CO)NCC(=O)O
• Canonical SMILES: OCC(NCC(=O)O)(CO)CO
• InChI: InChI=1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChIKey: SEQKRHFRPICQDD-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 2-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid
• IUPAC传统名: tricine
• 别名: N-三(羟甲基)甲基甘氨酸; 三(羟甲基)甲基甘氨酸; N-(三羟甲基甲基)甘氨酸; TRICINE, ELECTROPHORESIS GRADE; N-Tris-[hydroxymethyl]methylglycine; TRICINE; Tricine; N-(Tri(hydroxymethyl)methyl)glycine; Tricine; N-[Tris(hydroxymethyl)methyl]glycine; N-(Trishydroxymethylmethyl)glycine

登记号

• CAS号: 5704-04-1
• EC号: 227-193-6
• MDL号: MFCD00004277
• Beilstein号: 1937804
• 默克索引号: 149651
• PubChem SID: 24889851; 24899930; 24900603; 24899884; 162088290; 24900318
• PubChem CID: 79784
• CHEBI ID: 39063
• Chemspider ID: 72078
• Gmelin ID: 3688
• 医学主题词(MeSH): tricine
• 维基百科标题: Tricine

理论计算性质

• Acid pKa: 2.860766
• 质子受体: 6
• 质子供体: 5
• LogD (pH = 5.5): -5.245832
• LogD (pH = 7.4): -5.4276204
• Log P: -5.245555
• 摩尔折射率: 39.2147 cm^3
• 极化性: 15.894165 Å^3
• 极化表面积: 110.02 Å^2
• 可自由旋转的化学键: 6
• 里宾斯基五规则: true

分子性质

理化性质

• 溶解度: 89.6 g L^-1 (at 20 °C) in water; H2O: soluble0.5 M at 20 °C, clear; H2O: soluble0.5 M at 20 °C, clear, colorless; H2O: soluble1 M, clear, colorless
• 外观: White crystals
• 熔点: 179-189°C (dec.); 186-188 °C (dec.); 187 °C; ca 183°C dec.
• 紫外吸收波长: 260 nm; A1M/260, H2O ≤0.1; A1M/280, H2O ≤0.05; A20%/290 ≤0.02; λ: 260 nm Amax: 0.03; λ: 280 nm Amax: 0.02
• pKa: 8.1; 8.1 (25°C); 8.15 (20°C)
• 吸光度: 0.03
• pH值: 4.0-6.0 (1 M in H2O); 4.5-6.0 (25 °C, 0.5 M in H2O)

安全信息

• 保存条件: Room Temperature (15-30°C)
• 德国WGK号: 3
• TSCA收录: 是
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

产品相关信息

• 纯度: >99%; ≥99% (titration); ≥99.0% (NT); ≥99.5% (NT); 98%; 98+%
• 浓度: 1 M in solution
• 级别: Biotechnology Performance Certified; ELECTROPHORESIS
• 适用性: cell culture tested
• 燃烧残渣: ≤0.05% (as SO4); ≤0.1%
• 杂质: <5 ppm heavy metals as (Pb); ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter; bioburden, tested; DNase, RNase, Protease, none detected; endotoxin and bioburden, tested; endotoxin, tested; Insoluble matter, passes filter test
• 痕量阳离子: Al: ≤0.0005%; Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤0.0005%; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤0.0005%; Cu: ≤5 mg/kg; Fe: <5 ppm; Fe: ≤0.0005%; Fe: ≤5 mg/kg; heavy metals (as Pb): ≤5 ppm; K: ≤0.005%; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤0.0005%; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤0.005%; Na: ≤50 mg/kg; NH4+: ≤0.05%; Ni: ≤5 mg/kg; Pb: ≤0.001%; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤0.0005%; Zn: ≤5 mg/kg
• 痕量阴离子: chloride (Cl-): ≤0.1%; chloride (Cl-): ≤1000 mg/kg; chloride (Cl-): ≤500 mg/kg; sulfate (SO42-): ≤0.05%; sulfate (SO42-): ≤50 mg/kg
• 无菌消毒: 0.2 μm filtered
• 干燥失重: ≤0.5% loss on drying, 110 °C
• pH范围: 7.4 - 8.8
• 产品质量级别: ELITE; GMP
• λ: 0.5 M in H2O
• 适用pH范围: 7.4 - 8.8; 7.6 - 8.8
• 产品线: BioUltra
• 线性分子式: (HOCH2)3CNHCH2CO2H

详细说明

MP Biomedicals - 04807410

Electrophoresis Grade
Purity: >99%
Heavy metals (as Pb): <10 ppm
pKa at 25°C = 8.1
Biological buffer

MP Biomedicals - 02194556

(N-Tris-[hydroxymethyl]methylglycine) Cell Culture Reagent Biological buffer pKa at 25°C = 8.1 Buffering pH range 7.4-8.8

MP Biomedicals - 04807420

Electrophoresis Grade Purity: >99% Heavy metals (as Pb): <10 ppm pKa at 25°C = 8.1 Biological buffer

MP Biomedicals - 02103112

Biological buffer
pKa at 25°C = 8.1
Buffering pH range 7.4-8.8

MP Biomedicals - 04807416

Electrophoresis Grade Purity: >99% Heavy metals (as Pb): <10 ppm pKa at 25°C = 8.1 Biological buffer

MP Biomedicals - 04807413

Electrophoresis Grade Purity: >99% Heavy metals (as Pb): <10 ppm pKa at 25°C = 8.1 Biological buffer

Sigma Aldrich - 93356

Application
用于分离低分子量的肽的缓冲剂组分。
Other Notes
Buffer for biological research1; In the optimization of the firefly luciferase assay for ATP2; Component of a non-barbiturate buffer system for electrophoresis of serum proteins on cellulose acetate membranes3.

Sigma Aldrich - T0377

Application
用于分离低分子量的肽的缓冲剂组分。
包装
1 kg in poly bottle
25, 100, 250 g in poly bottle
5, 25 kg in poly drum

Sigma Aldrich - T1074

Application
用于分离低分子量的肽的缓冲剂组分。

Sigma Aldrich - T9784

Application
用于分离低分子量的肽的缓冲剂组分。

Sigma Aldrich - T5816

Application
用于分离低分子量的肽的缓冲剂组分。
Quality
经过内毒素和生物负载测试

Sigma Aldrich - 93355

Application
用于分离低分子量的肽的缓冲剂组分。
Other Notes
生物缓冲剂1

参考文献

• Biological buffer, pKa = 8.15 at 20^o: Biochemistry, 5, 467 (1966).