2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol

• ChemBase编号: 102295
• 分子式: C8H19NO5
• 平均质量: 209.24016
• 单一同位素质量: 209.12632271
• SMILES: OCCN(CCO)C(CO)(CO)CO
• Canonical SMILES: OCCN(C(CO)(CO)CO)CCO
• InChI: InChI=1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• InChIKey: OWMVSZAMULFTJU-UHFFFAOYSA-N

名称和登记号

名称

• IUPAC标准名: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
• IUPAC传统名: bis-tris; bis-tris buffer
• 别名: 2-二(2-羟乙基)氨基-2-羟甲基-1,3-丙二醇; 双(2-羟乙基)氨基-三(羟甲基)甲烷; 2,2-双(羟基甲基)-2,2',2''-次氮基三乙醇; 2-双(羟基甲基)-2,2′,2″-次氮基三乙醇; Bis(2-Hydroxyethyl)aminotris(hydroxymethyl)methane; Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane; Bis-TRIS; 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol; Bis-(2-hydroxy-ethyl)-amino-tris(hydroxymethyl)-methane; 2,2-Bis(hydroxymethyl)-2,2,2-nitrilotriethanol; Bis-tris methane; 2,2-Bis(hydroxymethyl)-2,2',2"-nitrilotriethanol; 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol; Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane; bis-TRIS; 2,2-Bis-(hydroxymethyl)-2,2',2''-nitrilotriethanol; 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol; Bis-tris

登记号

• CAS号: 6976-37-0
• EC号: 230-237-7
• MDL号: MFCD00002853
• Beilstein号: 2205275
• PubChem SID: 24892155; 162088644; 24892003; 24848919; 24891775
• PubChem CID: 81462
• CHEBI ID: 41250
• Chemspider ID: 73505
• Gmelin ID: 4519
• 医学主题词(MeSH): Bistris
• 维基百科标题: Bis-tris_methane

理论计算性质

• Acid pKa: 14.119829
• 质子受体: 6
• 质子供体: 5
• LogD (pH = 5.5): -5.8113337
• LogD (pH = 7.4): -4.0409145
• Log P: -3.276805
• 摩尔折射率: 51.0094 cm^3
• 极化性: 20.21558 Å^3
• 极化表面积: 104.39 Å^2
• 可自由旋转的化学键: 8
• 里宾斯基五规则: true

分子性质

理化性质

• 溶解度: 209.2 g L^-1 (at 20 °C) in water; H2O: soluble1 M at 20 °C, clear, colorless; H2O: soluble1 M, clear, colorless
• 外观: White crystals
• 熔点: 102 - 103°C; 102-105°C; 104 °C(lit.); 104°C
• 紫外吸收波长: 280 nm; λ: 280 nm Amax: 0.15
• pKa: 6.46; 6.5 (25°C)
• pKb: 7.54
• 气味: Odourless
• 吸光度: 0.15
• pH值: 9.0-10.1 (20-25 °C, 1 M in H2O); 9.5-11.0 (25 °C, 1 M in H2O)

安全信息

• 保存条件: Room Temperature (15-30°C)
• RTECS编号: TY2900000
• 欧盟危险性物质标志: 刺激性(Irritant) (Xi)
• 德国WGK号: 3
• 危险公开号: 36/37/38; r36/37/38
• 安全公开号: 26-36; 26-37-60; s26, s36
• TSCA收录: 是
• GHS危险品标识: gHS exclamation mark; GHS07
• GHS警示词: WARNING; Warning
• NFPA704:
  

  0

  2

  0
• GHS危险声明: 315, 319, 335; H315-H319-H335
• GHS警示性声明: 261, 305+351+338; P261-P305 + P351 + P338; P261-P305+P351+P338-P302+P352-P321-P405-P501A
• 个人保护装置: dust mask type N95 (US), Eyeshields, Gloves

产品相关信息

• 纯度: ≥99%; ≥98%; ≥98% (titration); ≥98.0% (titration); ≥99.0% (NT); 98%; 98+%
• 适用性: cell culture tested; suitable for insect cell culture
• 燃烧残渣: ≤0.1%; ≤0.3%
• 杂质: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter; endotoxin and total aerobic microbial count, tested; insoluble matter, passes filter test
• 痕量阳离子: Al: ≤0.0005%; Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤0.0005%; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤0.0005%; Cu: ≤5 mg/kg; Fe: ≤0.0005%; Fe: ≤5 mg/kg; heavy metals (as Pb): ≤5 ppm; K: ≤0.005%; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤0.0005%; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤0.005%; Na: ≤50 mg/kg; NH4+: ≤0.05%; Ni: ≤5 mg/kg; Pb: ≤0.001%; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤0.0005%; Zn: ≤5 mg/kg
• 痕量阴离子: chloride (Cl-): ≤0.05%; chloride (Cl-): ≤50 mg/kg; sulfate (SO42-): ≤0.05%; sulfate (SO42-): ≤50 mg/kg
• 干燥失重: ≤0.5% loss on drying, 20 °C (HV)
• pH范围: 5.5 - 8.0
• 产品质量级别: ELITE
• λ: 1 M in H2O
• 适用pH范围: 5.8 - 7.2
• 产品线: BioReagent; BioUltra; BioXtra
• abs.: absorption1M/270 nm, H2O cut-off
• 线性分子式: (HOCH2CH2)2NC(CH2OH)3
• Empirical Formula (Hill Notation): C8H19NO5

详细说明

MP Biomedicals - 02194546

Cell Culture Reagent Purity: 99+% Zwitterionic buffer pKa = 6.5 at 25°C Useful pH range 5.8-7.2.

MP Biomedicals - 02101038

Purity: 99+%
Zwitterionic buffer
pKa = 6.5 at 25°C
Useful pH range 5.8-7.2.

Sigma Aldrich - B9754

Other Notes
很容易将 BIS-TRIS 产品规格与 BIS-TRIS 规格表进行比较。
包装
25 kg in poly drum

Sigma Aldrich - B7535

Other Notes
很容易将 BIS-TRIS 产品规格与 BIS-TRIS 规格表进行比较。

Sigma Aldrich - B4429

Other Notes
很容易将 BIS-TRIS 产品规格与 BIS-TRIS 规格表进行比较。

Sigma Aldrich - 14879

Other Notes
Effective buffer for the assay of creatine kinase1; Effectively protects hemoglobin during freeze-drying2; Catholyte in IEF of 2-D gel electrophoresis3.
Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table.
Application
BIS-TRIS can be used to study biological buffers. BIS-TRIS has been used in a study that synthesized ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials. BIS-TRIS has also been used in a study that synthesized and described the photochemical properties of oligo- ortho -azobenzenes.

参考文献

• Buffer: pH range 5.5 - 8.0.