disodium hexahydrate 4-nitrophenyl phosphate

• ChemBase编号: 102259
• 分子式: C6H16NNa2O12P
• 平均质量: 371.144041
• 单一同位素质量: 371.02055015
• SMILES: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP(=O)([O-])[O-])cc1
• Canonical SMILES: [O-][N+](=O)c1ccc(cc1)OP(=O)([O-])[O-].O.O.O.O.O.O.[Na+].[Na+]
• InChI: InChI=1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChIKey: KAKKHKRHCKCAGH-UHFFFAOYSA-L

名称和登记号

名称

• IUPAC标准名: disodium hexahydrate 4-nitrophenyl phosphate
• IUPAC传统名: dipotassium hexahydrate @4-nitrophenyl phosphate; disodium hexahydrate p-nitrophenyl phosphate
• 别名: 4-硝基苯磷酸二钠 六水合物; 4-硝基苯磷酸盐 二钠盐 六水合物; pNPP 二钠 六水合物; 对硝基苯磷酸二钠; 对硝基苯磷酸盐 二钠 六水合物; Sigma 104® 磷酸酶底物; 4-硝基苯磷酸盐 二钠盐 六水合物; pNPP 二钠盐 六水合物; p-Nitrophenyl phosphate disodium hexahydrate; 4-Nitrophenyl phosphate disodium salt hexahydrate; di-Sodium 4-nitrophenyl phosphate; Disodium 4-nitrophenyl phosphate hexahydrate; pNPP disodium hexahydrate; p-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE; Sigma 104® Phosphatase Substrate; 4-Nitrophenyl phosphate disodium salt hexahydrate; pNPP disodium salt hexahydrate; Phosphatase substrate; p-Nitrophenyl phosphate disodium; pNPP

登记号

• CAS号: 333338-18-4; 4264-83-9
• EC号: 224-246-5
• MDL号: MFCD00066288
• Beilstein号: 5324661
• PubChem SID: 24899454; 24898495; 24886301; 24898699; 24897592; 24897708; 24897898; 24886300; 24898714; 162089978; 24897580; 24898723
• PubChem CID: 2723710

理论计算性质

• Acid pKa: 1.777862
• 质子受体: 5
• 质子供体: 0
• LogD (pH = 5.5): -1.4257605
• LogD (pH = 7.4): -2.1587105
• Log P: 0.9557995
• 摩尔折射率: 43.9931 cm^3
• 极化性: 17.17706 Å^3
• 极化表面积: 118.24 Å^2
• 可自由旋转的化学键: 3
• 里宾斯基五规则: true

分子性质

理化性质

• 溶解度: H2O: soluble1 tablet/3 mL, clear, colorless to very faintly yellow (6 mM); H2O: soluble100 mg/mL; H2O: soluble25 mg/mL at 20 °C, clear, yellow-green
• 外观: powder; tablet; tablets; white to pale yellow crystalline
• 熔点: > 300°C; >300 °C(lit.)
• 荧光: λex 310 nm (pH 7.1); λex 399 nm (Reaction Product)

安全信息

• 保存条件: 0°C
• 德国WGK号: 3
• 个人保护装置: Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
• 保存温度: -20°C; 2-8°C

产品相关信息

• 纯度: ≥99%; ≥97%; ≥97.0% (NT); ≥99.0% (5 mg substrate per tablet, rest binding agent, TLC); ≥99.0% (enzymatic); 98%
• 级别: for enzyme immunoassay
• 适用性: suitable as substrate for phosphatase test; suitable for cell culture
• 简要说明: 5 mg substrate per tablet
• 杂质: ≤0.01% free nitrophenol; p-nitrophenol, essentially free
• 临床用途: for in vitro diagnostic use
• 产品质量级别: PREMIUM
• 产品线: BioReagent
• 线性分子式: O2NC6H4OP(O)(ONa)2 · 6H2O

详细说明

MP Biomedicals - 02100878

Disodium Salt
Hexahydrate
Purity: 99+%
White to pale yellow crystals
Free p-nitrophenol <.005%
Suitable for use as a substrate for alkaline and acid phosphatase.

Sigma Aldrich - 104100

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
法律信息
Sigma 104 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - P5994

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins1. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
Quantity
Contains 40 mg substrate per tablet

Sigma Aldrich - N2640

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
Quantity
Contains 15 mg substrate per tablet

Sigma Aldrich - P5869

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.

Sigma Aldrich - N4645

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。

Sigma Aldrich - S0942

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.

Sigma Aldrich - 71770

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。

Sigma Aldrich - N9389

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。

Sigma Aldrich - P4744

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
包装
1, 5, 10, 25, 50 g in glass bottle

Sigma Aldrich - N2765

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
Quantity
Contains 20 mg substrate per tablet

Sigma Aldrich - 71768

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。

Sigma Aldrich - P5744

Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.

Sigma Aldrich - 104105

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
包装
5mg 片剂
法律信息
Sigma 104 注册商标 Sigma-Aldrich Co. LLC

Sigma Aldrich - 73730

Application
酸性和碱性磷酸酶测定的显色底物。1,2反应生成可溶性黄色终产物。加入 3 N NaOH 可终止反应，然后在 405nm 处进行读取。
包装
package with 50 tablets