disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate

• ChemBase编号: 102209
• 分子式: C22H14N6Na2O9S2
• 平均质量: 616.4909
• 单一同位素质量: 616.00590662
• SMILES: [Na+].[Na+].Nc1c2c(O)c(/N=N/c3ccccc3)c(cc2cc(c1/N=N/c1ccc(cc1)[N+](=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-]
• Canonical SMILES: [O-][N+](=O)c1ccc(cc1)/N=N/c1c(N)c2c(cc1S(=O)(=O)[O-])cc(c(c2O)/N=N/c1ccccc1)S(=O)(=O)[O-].[Na+].[Na+]
• InChI: InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2
• InChIKey: AOMZHDJXSYHPKS-UHFFFAOYSA-L

名称和登记号

名称

• IUPAC标准名: disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate; disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
• IUPAC传统名: disodium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate; dipotassium 4-amino-5-hydroxy-3-[2-(4-nitrophenyl)diazen-1-yl]-6-(2-phenyldiazen-1-yl)naphthalene-2,7-disulfonate; disodium 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazen-1-yl]-6-[(E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate
• 别名: 萘黑10B; Naphthalene Black 10B; Amido Black 10B; NAPHTHOL BLUE BLACK; Amidoschwarz; Acidal Black 10B; Acidal Navy Blue 3BR; Naphthalene Black 10B (see talk page); Naphthol Blue Black; Buffalo Black NBR; C.I. 20470; Acid Black 1; AMIDO BLACK 10B; Amido Black 10B; Naphthol Blue Black; Naphthol Blue Black solution; Amido Black Staining Solution 2X

登记号

• CAS号: 1064-48-8
• EC号: 213-903-1
• MDL号: MFCD00004017
• Beilstein号: 5374263
• PubChem SID: 24897883; 24891210; 24897642; 162089582; 24851681
• PubChem CID: 9566044; 5359556
• Chemspider ID: 10660833
• 维基百科标题: Amido_black_10B
• Color Index Number: 20470

理论计算性质

• Acid pKa: -3.1733475
• 质子受体: 14
• 质子供体: 2
• LogD (pH = 5.5): 0.19101588
• LogD (pH = 7.4): 0.19059597
• Log P: 0.776995
• 摩尔折射率: 144.1538 cm^3
• 极化性: 53.10609 Å^3
• 极化表面积: 255.91 Å^2
• 可自由旋转的化学键: 7
• 里宾斯基五规则: false

分子性质

理化性质

• 溶解度: ~ 30 g/l at 20 °C in water
• 外观: Dark red to black
• 闪点: 30 °C; 86 °F
• 紫外吸收波长: λmax 618 nm

安全信息

• 保存条件: Room Temperature (15-30°C)
• 保存注意事项: Hygroscopic
• RTECS编号: QJ6196000
• 欧盟危险性物质标志: 刺激性(Irritant) (Xi)
• 联合国危险货物编号: 2924
• 德国WGK号: 2; 3
• 联合国危险货物等级: 3
• 联合国危险货物包装类别(PG): 3
• 危险公开号: 10-36/38-67; 36/37/38; R:36/37/38; R20, R21, R33
• 安全公开号: 26; 26-37; S:20-25-26-37/39; S28A, S37, S45
• TSCA收录: 是
• GHS危险品标识: GHS02; GHS07
• GHS警示词: Warning
• NFPA704:
  

  0

  2

  0
• GHS危险声明: H226-H315-H319-H336; H315-H319-H335
• GHS警示性声明: P261-P305 + P351 + P338; P261-P305+P351+P338-P302+P352-P321-P405-P501A
• 个人保护装置: dust mask type N95 (US), Eyeshields, Gloves
• RID/ADR: UN 2924 3/PG 3

产品相关信息

• 纯度: ~50% (dye content); Dye Content ~85%
• 级别: for microscopy (Hist.)
• 主要成分: Dye content, ~50%; Dye content, ~85%; Dye content, 80%
• 适用性: suitable for electrophoresis
• 杂质: ≤10% water
• Empirical Formula (Hill Notation): C22H14N6Na2O9S2

详细说明

MP Biomedicals - 02100563

Useful dye for staining protein patterns after electrophoresis. Stains proteins, histones, orosomucoids. Also used on SDS-gels. Dye content: ~ 85%

MP Biomedicals - 04806717

General protein and nucleic acid stain for SDS-PAGE gels. Useful in staining not only proteins, but also histones and orosomucoid.

MP Biomedicals - 04806712

General protein and nucleic acid stain for SDS-PAGE gels. Useful in staining not only proteins, but also histones and orosomucoid.

Sigma Aldrich - A8181

Application
Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots).1 Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.2
Reconstitution
After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

Sigma Aldrich - N3393

Application
Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.
包装
25, 100 g in glass bottle

Sigma Aldrich - 195243

包装
25, 100 g in glass bottle